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. 1975 Jul;56(1):232–235. doi: 10.1172/JCI108073

The measurement of nephron filtration rate and absolute reabsorption in the proximal tubule of the rabbit kidney.

A M Chonko, R W Osgood, A E Nickel, T F Ferris, J H Stein
PMCID: PMC436574  PMID: 1141436

Abstract

Micropuncture studies were performed in the rabbit to determine nephron filtration rate and absolute fluid reabsorption in the proximal tubule in order to compare the latter value with data obtained with the in vitro microperfusion technique. New Zealand white rabbits, 2-2.8 kg, were studied. Nephron filtration rate was 21 nl/min (n equal to 48) and absolute reabsorption along the length of the accesible portion of the proximal convoluted tubule was 10.3 nl/min. Correcting this value for tubular length gives a fluid reabsorption of approximately 1.9 nl/mm per min. In view of the marked difference between the in vivo and in vitro techniques and the various sources of error with each, this is reasonably similiar to the value of 1.3 nl/mm per min obtained in the isolated proximal convoluted tubule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bankir L., Farman N., Grünfeld J. P., De la Tour E. H., Funck-Brentano J. L. Radioactive microsphere distribution and single glomerular blood flow in the normal rabbit kidney. Pflugers Arch. 1973 Aug 17;342(2):111–123. doi: 10.1007/BF00587842. [DOI] [PubMed] [Google Scholar]
  2. Burg M. B., Green N. Function of the thick ascending limb of Henle's loop. Am J Physiol. 1973 Mar;224(3):659–668. doi: 10.1152/ajplegacy.1973.224.3.659. [DOI] [PubMed] [Google Scholar]
  3. Burg M. B., Orloff J. Control of fluid absorption in the renal proximal tubule. J Clin Invest. 1968 Sep;47(9):2016–2024. doi: 10.1172/JCI105888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burg M., Grantham J., Abramow M., Orloff J. Preparation and study of fragments of single rabbit nephrons. Am J Physiol. 1966 Jun;210(6):1293–1298. doi: 10.1152/ajplegacy.1966.210.6.1293. [DOI] [PubMed] [Google Scholar]
  5. Cortell S. Silicone rubber for renal tubular injection. J Appl Physiol. 1969 Jan;26(1):158–159. doi: 10.1152/jappl.1969.26.1.158. [DOI] [PubMed] [Google Scholar]
  6. FUHR J., KACZMARCZYK J., KRUTTGEN C. D. Eine einfache colorimetrische Methode zur Inulinbestimmung für Nieren-Clearance-Untersuchungen bei Stoffwechselgesunden und Diabetikern. Klin Wochenschr. 1955 Aug 1;33(29-30):729–730. doi: 10.1007/BF01473295. [DOI] [PubMed] [Google Scholar]
  7. Grantham J. J., Kurg M. B., Obloff J. The nature of transtubular Na and K transport in isolated rabbit renal collecting tubules. J Clin Invest. 1970 Oct;49(10):1815–1826. doi: 10.1172/JCI106399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hamburger R. J., Lawson N. L., Dennis V. W. Effects of cyclic adenosine nucleotides on fluid absorption by different segments of proximal tubule. Am J Physiol. 1974 Aug;227(2):396–401. doi: 10.1152/ajplegacy.1974.227.2.396. [DOI] [PubMed] [Google Scholar]
  9. Horster M., Burg M., Potts D., Orloff J. Fluid absorption by proximal tubules in the absence of a colloid osmotic gradient. Kidney Int. 1973 Jul;4(1):6–11. doi: 10.1038/ki.1973.74. [DOI] [PubMed] [Google Scholar]
  10. Imai M., Kokko J. P. Effect of peritubular protein concentration on reabsorption of sodium and water in isolated perfused proxmal tubules. J Clin Invest. 1972 Feb;51(2):314–325. doi: 10.1172/JCI106816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Imai M., Kokko J. P. Sodium chloride, urea, and water transport in the thin ascending limb of Henle. Generation of osmotic gradients by passive diffusion of solutes. J Clin Invest. 1974 Feb;53(2):393–402. doi: 10.1172/JCI107572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kokko J. P. Sodium chloride and water transport in the descending limb of Henle. J Clin Invest. 1970 Oct;49(10):1838–1846. doi: 10.1172/JCI106401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rocha A. S., Kokko J. P. Sodium chloride and water transport in the medullary thick ascending limb of Henle. Evidence for active chloride transport. J Clin Invest. 1973 Mar;52(3):612–623. doi: 10.1172/JCI107223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stein J. H., Congbalay R. C., Karsh D. L., Osgood R. W., Ferris T. F. The effect of bradykinin on proximal tubular sodium reabsorption in the dog: evidence for functional nephron heterogeneity. J Clin Invest. 1972 Jul;51(7):1709–1721. doi: 10.1172/JCI106972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stein J. H., Osgood R. W., Boonjarern S., Ferris T. F. A comparison of the segmental analysis of sodium reabsorption during Ringer's and hyperoncotic albumin infusion in the rat. J Clin Invest. 1973 Sep;52(9):2313–2323. doi: 10.1172/JCI107420. [DOI] [PMC free article] [PubMed] [Google Scholar]

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