Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Angiogenesis. 2014 Nov 25;18(2):137–150. doi: 10.1007/s10456-014-9451-4

Figure-5. Candesartan stimulated VEGFR2 activation but did not alter VEGF level in hypoxia.

Figure-5

(A) Gene expression of VEGF mRNA normalized to 18SmRNA of four-groups. (B-C) Representative images of Western blot and statistical analysis for the protein expression of VEGF normalized to β-actin. Candesartan treatment and hypoxia significantly increased the protein expression of VEGF compared to normoxia. Treatment with candesartan during hypoxia did not alter VEGF expression compared to hypoxia. (D-E) Representative image of Western blot and statistical analysis for VEGFR2 activation showing that hypoxia induced phosphorylation of VEGFR2, an effect that was further maintained by candesartan. Two-way ANOVA showed a significant interaction between candesartan and hypoxia in retinal VEGFR2 activation and VEGF mRNA levels but not at VEGF protein level, *p<0.05, n=4-7/group.