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. 1976 Feb;57(2):530–533. doi: 10.1172/JCI108307

Apoprotein B in fasting and postprandial human jejunal mucosa.

D Rachmilewitz, J J Albers, D R Saunders
PMCID: PMC436680  PMID: 1254733

Abstract

We tested whether apoprotein B is present in fasting and postprandial human duodenojejunal mucosa because lipoprotein-like particles are visualized by electron microscopy within the smooth endoplasmic reticulum and the Golgi cisternae of these absorptive cells. Duodenojejunal biopsies from normal volunteers were incubated in citrate buffer and were shaken in 1% EDTA so that absorptive cells could be freed from underlying tissue. Apoprotein B was determined by double-antibody radioimmunoassay in homogenates of absorptive cells. The preparations of absorptive cells were shown to be uncontaminated by plasma lipoproteins; they did not contain any albumin by immunodiffusion able to detect 2 mug/ml. They adsorbed less than 0.1% of 125I-low density lipoprotein which was added to the citrate buffer. Cell preparations from suction biopsies of human rectum contained no detectable apoprotein B. Duodenojejunal absorptive cells from 22 fasting subjects contained 3.2 +/- 0.5 mug of apoprotein B per 100 mg (wet wt) of biopsies or 1.3 mug of apoprotein B per mg of total cell protein. The amount of apoprotein B per milligram of cell protein fell to 0.3 mug in 14 of these individuals whose mucosa was also sampled 45 min after instilling fat intraduodenally. These experiments provide immunochemical evidence that human duodenojejunal absorptive cells contain apoprotein B. This technique should be valuable for studying the physiology of intestinal lipoproteins in absorption and in patients with hyperlipidemia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albers J. J., Cabana V. G., Hazzard W. R. Immunoassay of human plasma apolipoprotein B. Metabolism. 1975 Dec;24(12):1339–1351. doi: 10.1016/0026-0495(75)90050-5. [DOI] [PubMed] [Google Scholar]
  2. DenBesten L., Reyna R. H., Connor W. E., Stegink L. D. The different effects on the serum lipids and fecal steroids of high carbohydrate diets given orally or intravenously. J Clin Invest. 1973 Jun;52(6):1384–1393. doi: 10.1172/JCI107311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FLICK A. L., QUINTON W. E., RUBIN C. E. A peroral hydraulic biopsy tube for multiple sampling at any level of the gastro-intestinal tract. Gastroenterology. 1961 Jan;40:120–126. [PubMed] [Google Scholar]
  4. FLICK A. L., VOEGTLIN K. F., RUBIN C. E. Clinical experience with suction biopsy of the rectal mucosa. Gastroenterology. 1962 Jun;42:691–705. [PubMed] [Google Scholar]
  5. Gotto A. M., Brown W. V., Levy R. I., Birnbaumer M. E., Fredrickson D. S. Evidence for the identity of the major apoprotein in low density and very low density lipoproteins in normal subjects and patients with familial hyperlipoproteinemia. J Clin Invest. 1972 Jun;51(6):1486–1494. doi: 10.1172/JCI106945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ISSELBACHER K. J., BUDZ D. M. SYNTHESIS OF LIPOPROTEINS BY RAT INTESTINAL MUCOSA. Nature. 1963 Oct 26;200:364–365. doi: 10.1038/200364b0. [DOI] [PubMed] [Google Scholar]
  7. ISSELBACHER K. J., SCHEIG R., PLOTKIN G. R., CAULFIELD J. B. CONGENITAL BETA-LIPOPROTEIN DEFICIENCY: AN HEREDITARY DISORDER INVOLVING A DEFECT IN THE ABSORPTION AND TRANSPORT OF LIPIDS. Medicine (Baltimore) 1964 May;43:347–361. [PubMed] [Google Scholar]
  8. Jones A. L., Ockner R. K. An electron microscopic study of endogenous very low density lipoprotein production in the intestine of rat and man. J Lipid Res. 1971 Sep;12(5):580–589. [PubMed] [Google Scholar]
  9. Kessler J. I., Stein J., Dannacker D., Narcessian P. Biosynthesis of low density lipoprotein by cell-free preparations of rat intestinal mucosa. J Biol Chem. 1970 Oct 25;245(20):5281–5288. [PubMed] [Google Scholar]
  10. Kostner G., Holasek A. Characterization and quantitation of the apolipoproteins from human chyle chylomicrons. Biochemistry. 1972 Mar 28;11(7):1217–1223. doi: 10.1021/bi00757a016. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Mistilis S. P., Ockner R. K. Effects of ethanol on endogenous lipid and lipoprotein metabolism in small intestine. J Lab Clin Med. 1972 Jul;80(1):34–46. [PubMed] [Google Scholar]
  13. Ockner R. K., Bloch K. J., Isselbacher K. J. Very-low-density lipoprotein in intestinal lymph: evidence for presence of the A protein. Science. 1968 Dec 13;162(3859):1285–1286. doi: 10.1126/science.162.3859.1285. [DOI] [PubMed] [Google Scholar]
  14. Ockner R. K., Hughes F. B., Isselbacher K. J. Very low density lipoproteins in intestinal lymph: origin, composition, and role in lipid transport in the fasting state. J Clin Invest. 1969 Nov;48(11):2079–2088. doi: 10.1172/JCI106174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rachmilewitz D., Stein O., Roheim P. S., Stein Y. Metabolism of iodinated high density lipoproteins in the rat. II. Autoradiographic localization in the liver. Biochim Biophys Acta. 1972 Jul 7;270(3):414–425. doi: 10.1016/0005-2760(72)90204-4. [DOI] [PubMed] [Google Scholar]
  16. Roheim P. S., Gidez L. I., Eder H. A. Extrahepatic synthesis of lipoproteins of plasma and chyle: role of the intestine. J Clin Invest. 1966 Mar;45(3):297–300. doi: 10.1172/JCI105343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rubin C. E. Electron microscopic studies of triglyceride absorption in man. Gastroenterology. 1966 Jan;50(1):65–77. [PubMed] [Google Scholar]
  18. Saunders D. R., Ways P. O., Parmentier C. M., Rubin C. E. Studies on the lipid composition of human small bowel mucosa. J Clin Invest. 1966 Sep;45(9):1516–1525. doi: 10.1172/JCI105458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shore B., Shore V. Isolation and characterization of polypeptides of human serum lipoproteins. Biochemistry. 1969 Nov;8(11):4510–4516. doi: 10.1021/bi00839a043. [DOI] [PubMed] [Google Scholar]
  20. Stern B. K., Jensen W. E. Active transport of glucose by suspensions of isolated rat intestinal epithelial cells. Nature. 1966 Feb 19;209(5025):789–790. doi: 10.1038/209789a0. [DOI] [PubMed] [Google Scholar]
  21. Tytgat G. N., Rubin C. E., Saunders D. R. Synthesis and transport of lipoprotein particles by intestinal absorptive cells in man. J Clin Invest. 1971 Oct;50(10):2065–2078. doi: 10.1172/JCI106700. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Windmueller H. G., Herbert P. N., Levy R. I. Biosynthesis of lymph and plasma lipoprotein apoproteins by isolated perfused rat liver and intestine. J Lipid Res. 1973 Mar;14(2):215–223. [PubMed] [Google Scholar]
  23. Windmueller H. G., Levy R. I. Production of beta-lipoprotein by intestine in the rat. J Biol Chem. 1968 Sep 25;243(18):4878–4884. [PubMed] [Google Scholar]

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