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The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1976 Apr;57(4):836–841. doi: 10.1172/JCI108359

Elaboration of toxic oxygen by-products by neutrophils in a model of immune complex disease.

R B Johnston Jr, J E Lehmeyer
PMCID: PMC436726  PMID: 181401

Abstract

Contact between human neutrophils and aggregated immunoglobulin G bound to micropore filters has been studied as a model of the pathogenesis of tissue damage in immune complex disease. Contact with this surface, as well as with plain filters and polystyrene petri dishes, induced neutrophils to elaborate superoxide anion and hydrogen peroxide and to generate chemiluminescence, which has been attributed to singlet oxygen. Pretreatment of the cells with cytochalasin B decreased these activities but increased release of lysosomal beta-glucuronidase, suggesting that degranulation and the burst of oxygen metabolism that characterizes phagocytes are independently regulated functions. Toxic oxygen metabolites released from neutrophils are highly reactive and could mediate tissue injury at sites of inflammation.

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Selected References

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