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. 1976 May;57(5):1221–1226. doi: 10.1172/JCI108390

Location of phosphatidylethanolamine and phosphatidylserine in the human platelet plasma membrane.

P K Schick, K B Kurica, G K Chacko
PMCID: PMC436775  PMID: 1262468

Abstract

The location of phospholipids in the human platelet plasma membrane was probed with 2, 4, 6-trinitrobenzenesulfonate (TNBS). TNBS does not penetrate inintact cells and can label phosphatidylethanolamine (PE) and phosphatidylserine (PS). In tact platelets, PE is not accessible to TNBS during the initial 15 min. However, 6.9% PE reacts with TNBS after 30 min and 17.9% PE is labeled after 90 min. In intact platelets, PS is not labeled even after 2 h. In contrast, in phospholipids extracted from platelets 71% PE and 26.5% PS react with TNBS within 5 min. This indicates that PS is inaccessible and PE is relatively inaccessible to TNBS in intact platelets. After incubation of platelets with thrombin, there is increased labeling of PE but no labeling of PS. The incubation of platelets with thrombin (0.05 U/ml) for 5 min results in 16.2% increase of PE labeling during subsequent 30-min incubation with TNBS. PS does not appear to be a component of the functional surface of platelets. However, exposure of PE may have a critical role in platelet hemostatic function. The implication of the study is that there is asymmetry of phopholipids in the platelet plasma membrane which has considerable physiological significance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Barber A. J., Jamieson G. A. Isolation and characterization of plasma membranes from human blood platelets. J Biol Chem. 1970 Dec 10;245(23):6357–6365. [PubMed] [Google Scholar]
  3. Bonsall R. W., Hunt S. Reactivity of the human erythrocyte membrane to sodium trinitrobenzenesulphonate. Biochim Biophys Acta. 1971 Oct 12;249(1):281–284. doi: 10.1016/0005-2736(71)90105-2. [DOI] [PubMed] [Google Scholar]
  4. Deykin D., Snyder D. Effect of epinephrine on platelet lipid metabolism. J Lab Clin Med. 1973 Oct;82(4):554–559. [PubMed] [Google Scholar]
  5. Feagler J. R., Tillack T. W., Chaplin D. D., Majerus P. W. The effects of thrombin on phytohemagglutinin receptor sites in human platelets. J Cell Biol. 1974 Mar;60(3):541–553. doi: 10.1083/jcb.60.3.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gordesky S. E., Marinetti G. V., Love R. The reaction of chemical probes with the erythrocyte membrane. J Membr Biol. 1975;20(1-2):111–132. doi: 10.1007/BF01870631. [DOI] [PubMed] [Google Scholar]
  7. Gordesky S. E., Marinetti G. V. The asymetric arrangement of phospholipids in the human erythrocyte membrane. Biochem Biophys Res Commun. 1973 Feb 20;50(4):1027–1031. doi: 10.1016/0006-291x(73)91509-x. [DOI] [PubMed] [Google Scholar]
  8. Holmsen H., Weiss H. J. Hereditary defect in the platelet release reaction caused by a deficiency in the storage pool of platelet adenine nucleotides. Br J Haematol. 1970 Nov;19(5):643–649. doi: 10.1111/j.1365-2141.1970.tb01648.x. [DOI] [PubMed] [Google Scholar]
  9. Lewis N., Majerus P. W. Lipid metabolism in human platelets. II. De novo phospholipid synthesis and the effect of thrombin on the pattern of synthesis. J Clin Invest. 1969 Nov;48(11):2114–2123. doi: 10.1172/JCI106178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lloyd J. V., Nishizawa E. E., Haldar J., Mustard J. F. Changes in 32 p-labelling of platelet phospholipids in response to ADP. Br J Haematol. 1972 Nov;23(5):571–585. doi: 10.1111/j.1365-2141.1972.tb07092.x. [DOI] [PubMed] [Google Scholar]
  11. Lundblad R. L. A rapid method for the purification of bovine thrombin and the inhibition of the purified enzyme wtih phenylmethylsulfonyl fluoride. Biochemistry. 1971 Jun 22;10(13):2501–2506. doi: 10.1021/bi00789a012. [DOI] [PubMed] [Google Scholar]
  12. Majerus P. W., Brodie G. N. The binding of phytohemagglutinins to human platelet plasma membranes. J Biol Chem. 1972 Jul 10;247(13):4253–4257. [PubMed] [Google Scholar]
  13. Marcus A. J. The role of lipids in blood coagulation. Adv Lipid Res. 1966;4:1–37. [PubMed] [Google Scholar]
  14. Marcus A. J., Ullman H. L., Safier L. B. Lipid composition of subcellular particles of human blood platelets. J Lipid Res. 1969 Jan;10(1):108–114. [PubMed] [Google Scholar]
  15. Marcus A. J., Zucker-Franklin D., Safier L. B., Ullman H. L. Studies on human platelet granules and membranes. J Clin Invest. 1966 Jan;45(1):14–28. doi: 10.1172/JCI105318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Means G. E., Congdon W. I., Bender M. L. Reactions of 2,4,6-trinitrobenzenesulfonate ion with amines and hydroxide ion. Biochemistry. 1972 Sep 12;11(19):3564–3571. doi: 10.1021/bi00769a011. [DOI] [PubMed] [Google Scholar]
  17. Nachman R. L., Hubbard A., Ferris B. Iodination of the human platelet membrane. Studies of the major surface glycoprotein. J Biol Chem. 1973 Apr 25;248(8):2928–2936. [PubMed] [Google Scholar]
  18. Phillips D. R., Agin P. P. Thrombin substrates and the proteolytic site of thrombin action on human-platelet plasma membranes. Biochim Biophys Acta. 1974 Jun 13;352(2):218–227. doi: 10.1016/0005-2736(74)90213-2. [DOI] [PubMed] [Google Scholar]
  19. Phillips D. R. Effect of trypsin on the exposed polypeptides and glycoproteins in the human platelet membrane. Biochemistry. 1972 Nov 21;11(24):4582–4588. doi: 10.1021/bi00774a025. [DOI] [PubMed] [Google Scholar]
  20. Reimers H. J., Allen D. J., Feuerstein I. A., Mustard J. F. Transport and storage of serotonin by thrombin-treated platelets. J Cell Biol. 1975 May;65(2):359–372. doi: 10.1083/jcb.65.2.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rouser G., Siakotos A. N., Fleischer S. Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots. Lipids. 1966 Jan;1(1):85–86. doi: 10.1007/BF02668129. [DOI] [PubMed] [Google Scholar]
  22. Schick P. K., Spaet T. H., Jaffé E. R. The effects of phenazinemethosulfate and methylene blue on human platelet phospholipid synthesis. Proc Soc Exp Biol Med. 1972 Oct;141(1):114–118. doi: 10.3181/00379727-141-36728. [DOI] [PubMed] [Google Scholar]
  23. Schick P. K., Yu B. P. Methylene blue-induced serotonin release in human platelets. J Lab Clin Med. 1973 Oct;82(4):546–553. [PubMed] [Google Scholar]
  24. Schick P. K., Yu B. P. The role of platelet membrane phospholipids in the platelet release reaction. J Clin Invest. 1974 Nov;54(5):1032–1039. doi: 10.1172/JCI107846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Singer S. J. The molecular organization of membranes. Annu Rev Biochem. 1974;43(0):805–833. doi: 10.1146/annurev.bi.43.070174.004105. [DOI] [PubMed] [Google Scholar]

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