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. 2015 Mar 9;144(4):611–620. doi: 10.1111/imm.12412

Figure 2.

Figure 2

The lifespan of CD16− and CD16+ monocytes is regulated by caspase-3. CD16− and CD16+ monocytes were cultured for the indicated times with 1 or 25 μm DEVD-FMK (DEVD) or diluent control DMSO (−). (a) Percentage of apoptotic cells was assessed by Annexin V/7-AAD staining. (b) Percentage of cells stained with an anti-active-caspase-3-phycoerythrin antibody. (c) The activity of caspase-3 was evaluated using the DEVD-AFC assay. (d) Percentage of cells stained with the anti-cleaved PARP-FITC antibody. Data represent mean ± SEM (n = 3, *P < 0·05, **P < 0·01, ***P < 0·001).