Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1983 Feb;71(2):404–407. doi: 10.1172/JCI110784

Circulating Bovine Lymphocytes Contain Receptors for Parathyroid Hormone

Itsuo Yamamoto 1, John T Potts Jr 1, Gino V Segre 1
PMCID: PMC436882  PMID: 6296202

Abstract

No cell type practicably obtainable in vivo, such as blood cells, is known to contain parathyroid hormone (PTH) receptors; this deficiency has hampered investigation of receptor regulation. Second, PTH in vivo is among the potent stimulators of osteoclastic activity, although no direct hormonal effects on these cells have been identified. Several lines of evidence suggest that cells of the immune system may mediate PTH effects on osteoclasts. We, therefore, studied bovine blood cells for the presence of PTH receptors and PTH-stimulated adenylate cyclase. Using an analogue of bovine PTH, 125I-labeled [Nle8,Nle18,Tyr34]bPTH-(1--34)amide, we found PTH-specific binding to intact, nonadherent mononuclear cells (lymphocytes) and PTH-stimulated adenylate cyclase in plasma membranes prepared from these cells, and not with cells or membranes from other blood cells. Lymphocytes may serve to study the effects of physiologic and pathologic perturbations on PTH-receptor function in vivo. Exploration of PTH-related lymphocyte responses may help define the relation between cells of the immune system and osteoclastic bone resorption.

Full text

PDF

Images in this article

405Image
on p.405

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archer J. A., Gorden P., Gavin J. R., 3rd, Lesniak M. A., Roth J. Insulin receptors in human circulating lymphocytes: application to the study of insulin resistance in man. J Clin Endocrinol Metab. 1973 Apr;36(4):627–633. doi: 10.1210/jcem-36-4-627. [DOI] [PubMed] [Google Scholar]
  2. Bhathena S. J., Louie J., Schechter G. P., Redman R. S., Wahl L., Recant L. Identification of human mononuclear leukocytes bearing receptors for somatostatin and glucagon. Diabetes. 1981 Feb;30(2):127–131. doi: 10.2337/diab.30.2.127. [DOI] [PubMed] [Google Scholar]
  3. Dayer J. M., Passwell J. H., Schneeberger E. E., Krane S. M. Interactions among rheumatoid synovial cells and monocyte-macrophages: production of collagenase-stimulating factor by human monocytes exposed to concanavalin A or immunoglobulin Fc fragments. J Immunol. 1980 Apr;124(4):1712–1720. [PubMed] [Google Scholar]
  4. Dominguez J. H., Mundy G. R. Monocytes mediate osteoclastic bone resorption by prostaglandin production. Calcif Tissue Int. 1980;31(1):29–34. doi: 10.1007/BF02407164. [DOI] [PubMed] [Google Scholar]
  5. Gavin J. R., 3rd, Gorden P., Roth J., Archer J. A., Buell D. N. Characteristics of the human lymphocyte insulin receptor. J Biol Chem. 1973 Mar 25;248(6):2202–2207. [PubMed] [Google Scholar]
  6. Geier S. S., Cresswell P. Monocytes as accessory cells in the human mixed lymphocyte response: lack of requirement at high cell densities. Cell Immunol. 1980 Jan;49(1):190–195. doi: 10.1016/0008-8749(80)90068-4. [DOI] [PubMed] [Google Scholar]
  7. Goldstein S., Blecher M., Binder R., Perrino P. V., Recant L. Hormone receptors, 5. Binding of glucagon and insulin to human circulating mononuclear cells in diabetes mellitus. Endocr Res Commun. 1975;2(4-5):367–376. doi: 10.1080/07435807509089009. [DOI] [PubMed] [Google Scholar]
  8. Milhaud G., Labat M. L. Thymus and osteopetrosis. Clin Orthop Relat Res. 1978 Sep;(135):260–271. [PubMed] [Google Scholar]
  9. Minkin C., Blackman L., Newbrey J., Pokress S., Posek R., Walling M. Effects of parathyroid hormone and calcitonin on adenylate cyclase in murine mononuclear phagocytes. Biochem Biophys Res Commun. 1977 Jun 6;76(3):875–881. doi: 10.1016/0006-291x(77)91582-0. [DOI] [PubMed] [Google Scholar]
  10. Minkin C., Blackman L., Newbrey J., Pokress S., Posek R., Walling M. Effects of parathyroid hormone and calcitonin on adenylate cyclase in murine mononuclear phagocytes. Biochem Biophys Res Commun. 1977 Jun 6;76(3):875–881. doi: 10.1016/0006-291x(77)91582-0. [DOI] [PubMed] [Google Scholar]
  11. Rosenblatt M., Goltzman D., Keutmann H. T., Tregear G. W., Potts J. T., Jr Chemical and biological properties of synthetic, sulfur-free analogues of parathyroid hormone. J Biol Chem. 1976 Jan 10;251(1):159–164. [PubMed] [Google Scholar]
  12. Rosenfeld R., Thorsson A. V., Hintz R. L. Increased somatomedin receptor sites in newborn circulating mononuclear cells. J Clin Endocrinol Metab. 1979 Mar;48(3):456–461. doi: 10.1210/jcem-48-3-456. [DOI] [PubMed] [Google Scholar]
  13. Segre G. V., Rosenblatt M., Reiner B. L., Mahaffey J. E., Potts J. T., Jr Characterization of parathyroid hormone receptors in canine renal cortical plasma membranes using a radioiodinated sulfur-free hormone analogue. Correlation of binding with adenylate cyclase activity. J Biol Chem. 1979 Aug 10;254(15):6980–6986. [PubMed] [Google Scholar]
  14. Shortman K., Williams N., Jackson H., Russell P., Byrt P., Diener E. The separation of different cell classes from lymphoid organs. IV. The separation of lymphocytes from phagocytes on glass bead columns, and its effect on subpopulations of lymphocytes and antibody-forming cells. J Cell Biol. 1971 Mar;48(3):566–579. doi: 10.1083/jcb.48.3.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thorsson A. V., Hintz R. L. Specific 125I-somatomedin receptor on circulating human mononuclear cells. Biochem Biophys Res Commun. 1977 Feb 21;74(4):1566–1573. doi: 10.1016/0006-291x(77)90621-0. [DOI] [PubMed] [Google Scholar]
  16. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]
  17. Yoneda T., Mundy G. R. Monocytes regulate osteoclast-activating factor production by releasing prostaglandins. J Exp Med. 1979 Aug 1;150(2):338–350. doi: 10.1084/jem.150.2.338. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES