Figure 7. PcGs cooperate with Scrib module proteins to regulate growth.
(A–D) Knockdown of ph-p has little effect on WT growth but increases the growth of dlghypo tissue. Quantification is in E (***p < 0.0001). (F–G) BrmDN expression in dlgRNAi tissue decreases both upd3.3LacZ activation and overgrowth. (H) PcG components ph-p and Psc are downregulated in scrib and dlg mutant tissue (average in green), similar to levels observed upon JNK activation (blue). (**p < 0.005 ✝FDR < 0.05 in one genotype; ✝✝FDR < 0.05 in both genotypes) (I–K) Knockdown of ph-p or expression of a moderately active form of aPKC (aPKCmild) does not induce upd3.3LacZ, and aPKCmild induces only slight overgrowth. However, co-expression of these transgenes leads to strong overgrowth and upd3.3LacZ expression. Scale bar: 100 μm.