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Native PAGE analysis of c-E-helix-PYP–K-helix-F complexes. Coomassie staining (left) showing the migration pattern of the dimer and monomer. Fluorescence image (right) (488 nm excitation, 530 nm emission) showing that fluorescein-labeled K-peptide interacts with only the dimeric form of the protein. Protein concentrations were (i) 18.7, (ii) 7.5, (iii) 0.75, and (iv) 0.4 μM.
