Figure 2.

Deptor expression is regulated by ERα. (A) Deptor mRNA expression is significantly lower in ERα-negative (ERα-neg) breast cancer subtypes as compared to ERα-positive (ERα-pos) breast cancer subtypes as determined by the Broad-Novartis Cancer Cell Line Encyclopedia. Inset shows mean (± SEM) of Deptor mRNA expression in ERα-negative and ERα-positive breast cancer cells. (B) Oncomine analyses associated elevated Deptor mRNA expression levels with that of ERα-positive breast cancers using the Waddell Breast data set. (C) MCF7 cells were treated with tamoxifen (0.1 nM) or fulvestrant (ICI, 0.1 nM) for 4 days, at which point the cells were harvested and probed for Deptor expression by immunoblot analysis. MCF7 cells were treated with either estradiol (10 nM) or TGF-β1 (5 ng/ml) for 4 days before monitoring Deptor or ERα expression by immunoblot analysis. Immunoblots in C to E are representative of three independent experiments.