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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Pituitary. 2015 Apr;18(2):225–231. doi: 10.1007/s11102-014-0624-3

Fig. 1.

Fig. 1

Demonstration of dual corticotroph (assessed with ACTH, Tpit, and NeuroD1 markers) and gonadotroph (assessed with LH, DAX-1, and SF-1 markers) lineage in an SCA. Fluorescence immunohistochemistry of corticotroph markers ACTH, Tpit, NeuroD1, LH, DAX-1, and SF-1 were performed in a silent corticotroph adenoma. Tumor was fixed in 10% formalin, paraffin embedded, and stained with primary antibodies, with Alexa 488 secondary antibodies and visualized with confocal immunofluorescence microscopy at 20-40 x magnification. Tumor is (a) ACTH positive (b) NeuroD1 positive (c) SF-1 positive (d) Tpit negative, and (e) DAX-1 positive. (f) Tumor exhibits co-localization of ACTH and LH, showing potential derivation of SCAs from dual corticotroph-gonadotroph line. Portion of the field enlarged for detail (100 x) highlights the co-localization in a cell. [10]