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. 2015 Mar 27;9(5):052602. doi: 10.1063/1.4916508

FIG. 2.

FIG. 2.

Effect of oil on particle aggregation and cell viability. (a) Evaporation-induced change of weight percentage (W/W0 × 100%) of the mixture solution of droplets and oil. W0 is the weight of the mixture at t = 0 min, and W is the weight at t = n min (n = 5, 10, 15, 20, 25, 30, 35, 40, and 45). Inset: image of aggregated hydrogel particles after the removal of PFP (scale bar = 50 μm). (b) Evaporation-induced change of weight percentage (W/W0 × 100%) with time, when particles are collected in the DMEM cell culture medium. Inset: image of a centrifuge tube containing layers of culture medium, particles, and PFP (scale bar = 5 mm). (c) Percentage of living cells in hydrogel particles (N/N0 × 100%) at different culture time (t). N0 is the number of living cells at t = 0 h, and N is number of living cells at t = n hours (n = 24, 48, 72, 96, 120, 144, 168, 192, and 216). Error bar is presented as standard deviations of the mean, which is calculated based on three sets of independent experiments.