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. Author manuscript; available in PMC: 2016 Mar 17.
Published in final edited form as: Biochemistry. 2015 Mar 3;54(10):1943–1955. doi: 10.1021/bi5014358

Table 3.

Thermal shifts of saFabI water-channel variants upon ternary complex formation a, b, c

Wild-type saFabI saFabI D249A saFabI S189A saFabI Y147F
E 62.6 63.4 61.2 63.2
E-NADPH-CG400549 71.2 64.6 64.2 71.2
E-NADP+-PT119 70.4 63.6 61.8 72.2
a

Values correspond to Tm measurements (°C)

b

Experiments were performed in duplicate. Data variability is approximately ± 0.2 °C.

c

PT119 is a representative potent diphenyl ether 13, whereas CG400549 is a representative potent 2-pyridone 34.