Table 3.
Protein | Ligand | N | Ka (×106) | Kd (nM) | ΔH (Kcal/mol) | ΔS (cal/mol/deg) |
---|---|---|---|---|---|---|
Wild-type PH-TH | IP6 | 1.1 ± 0.1 | 4.2 ± 0.3 | 238 ± 32 | −1.1 ± 0.2 | 26.7 |
Wild-type PHTH | IP4 | 0.9 ± 0.1 | 39 ± 11 | 26 ± 6 | 4.4 ± 0.1 | 49.5 |
PH-TH R28C/D24N | IP6 | 0.7 ± 0.2 | 0.21 ± 0.03 | 4760 ± 700 | −1.7 ± 0.6 | 17.5 |
The integrated heat from representative isothermal titration calorimetry experiments was fit with a one-binding site model. See methods for the details of data analysis.