(
A) Representative images of HeLa EGFR-GFP BAC cells after
continuous stimulation with 10 ng/ml EGF for the indicated time points.
EGFR-GFP fluorescence is shown in green, the corrected p-EGFR intensity is
shown in red, and DAPI-stained nuclei are shown in blue. Measurements from
each individual endosome were used for all quantifications. Scale bars, 10
μm. (
B–
C) Time course of histogram
distributions of the total p-EGFR (
B) or EGFR (
C)
integral intensity per endosome upon EGF stimulation as in
Figure 1. The histogram shows the number
of vesicles per 1000 μm
2 of the area covered by cells.
Intensity values were scaled by the mode of the histogram at 10 min. In all
graphs, experimental points were fitted with a log-normal distribution.
Points show the mean from three independent experiments with a total of
∼150 cells per time point or condition.