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. 1994 May 24;91(11):4703–4707. doi: 10.1073/pnas.91.11.4703

Subunit dynamics in Escherichia coli preprotein translocase.

J C Joly 1, M R Leonard 1, W T Wickner 1
PMCID: PMC43856  PMID: 8197122

Abstract

SecY, SecE, and band 1 copurify as the SecY/E integral membrane domain of Escherichia coli preprotein translocase. To measure the in vivo association of these polypeptides and assay possible exchange, plasmid-borne secY and secE genes were placed under control of the ara regulon and fused to DNA encoding the influenza hemagglutinin epitope. Cells were incubated with [35S]methionine, grown for a "chase" period, and then induced with arabinose to express epitope-tagged, nonradioactive SecY and SecE. Both the wild-type and epitope-tagged polypeptides assembled into functional, heterotrimeric SecY/E complex. However, immunoprecipitation with antibody to the epitope tag did not cross-precipitate radiolabeled SecY or SecE. Thus, these subunits normally associate stably in vivo.

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Selected References

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