Figure 8. Ectopic expression of Vav1 allows modulation of β-catenin in lung cancer cells.
a. Total cell extracts from stable H358 cells expressing control vector only, wt-Vav1 or the D797N mutant were analysed by immunoblotting with the indicated Abs. Fold increase of phospho-Y654- and phospho-S675-β-catenin in cells expressing wt-Vav1 and D797N compared to control cells were normalized to total β-catenin. b. Wt-Vav1 and D797N expressing cells proliferation. 2.105 stable H358 cells (left panel) or transiently transfected A549 cells (right panel) expressing the indicated constructs were seeded in 6-wells plate in duplicate and counted at the indicated times. Each value represents the mean of the cell number ± standard deviation from one representative experiment of a total of three. c. Morphology of H358 cells. Stable H358 cells expressing the indicated constructs were cultured below sub-confluence and photographed by phase contrast (magnification. 20x). d. Immunofluorescence analysis of β-catenin in H358 cell lines. The indicated stable H358 cell lines were analysed after anti-pS675β-catenin and Vav1 immunostaining (left panels) or anti- β-catenin and Vav1 immunostaining (right panel) by confocal microscopy.