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. 1994 May 24;91(11):4736–4740. doi: 10.1073/pnas.91.11.4736

Simulated responses of cerebellar Purkinje cells are independent of the dendritic location of granule cell synaptic inputs.

E De Schutter 1, J M Bower 1
PMCID: PMC43863  PMID: 8197127

Abstract

Cerebellar Purkinje cell responses to granule cell synaptic inputs were examined with a computer model including active dendritic conductances. Dendritic P-type Ca2+ channels amplified postsynaptic responses when the model was firing at a physiological rate. Small synchronous excitatory inputs applied distally on the large dendritic tree resulted in somatic responses of similar size to those generated by more proximal inputs. In contrast, in a passive model the somatic postsynaptic potentials to distal inputs were 76% smaller. The model predicts that the somatic firing response of Purkinje cells is relatively insensitive to the exact dendritic location of synaptic inputs. We describe a mechanism of Ca2+-mediated synaptic amplification, based on the subspiking threshold recruitment of P-type Ca2+ channels in the dendritic branches surrounding the input site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amitai Y., Friedman A., Connors B. W., Gutnick M. J. Regenerative activity in apical dendrites of pyramidal cells in neocortex. Cereb Cortex. 1993 Jan-Feb;3(1):26–38. doi: 10.1093/cercor/3.1.26. [DOI] [PubMed] [Google Scholar]
  2. Armstrong D. M., Rawson J. A. Activity patterns of cerebellar cortical neurones and climbing fibre afferents in the awake cat. J Physiol. 1979 Apr;289:425–448. doi: 10.1113/jphysiol.1979.sp012745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benardo L. S., Masukawa L. M., Prince D. A. Electrophysiology of isolated hippocampal pyramidal dendrites. J Neurosci. 1982 Nov;2(11):1614–1622. doi: 10.1523/JNEUROSCI.02-11-01614.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bernander O., Douglas R. J., Martin K. A., Koch C. Synaptic background activity influences spatiotemporal integration in single pyramidal cells. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11569–11573. doi: 10.1073/pnas.88.24.11569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bower J. M., Woolston D. C. Congruence of spatial organization of tactile projections to granule cell and Purkinje cell layers of cerebellar hemispheres of the albino rat: vertical organization of cerebellar cortex. J Neurophysiol. 1983 Mar;49(3):745–766. doi: 10.1152/jn.1983.49.3.745. [DOI] [PubMed] [Google Scholar]
  6. De Schutter E., Bower J. M. An active membrane model of the cerebellar Purkinje cell II. Simulation of synaptic responses. J Neurophysiol. 1994 Jan;71(1):401–419. doi: 10.1152/jn.1994.71.1.401. [DOI] [PubMed] [Google Scholar]
  7. De Schutter E., Bower J. M. An active membrane model of the cerebellar Purkinje cell. I. Simulation of current clamps in slice. J Neurophysiol. 1994 Jan;71(1):375–400. doi: 10.1152/jn.1994.71.1.375. [DOI] [PubMed] [Google Scholar]
  8. Farrant M., Cull-Candy S. G. Excitatory amino acid receptor-channels in Purkinje cells in thin cerebellar slices. Proc Biol Sci. 1991 Jun 22;244(1311):179–184. doi: 10.1098/rspb.1991.0067. [DOI] [PubMed] [Google Scholar]
  9. Gruol D. L., Jacquin T., Yool A. J. Single-channel K+ currents recorded from the somatic and dendritic regions of cerebellar Purkinje neurons in culture. J Neurosci. 1991 Apr;11(4):1002–1015. doi: 10.1523/JNEUROSCI.11-04-01002.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Harvey R. J., Napper R. M. Quantitative studies on the mammalian cerebellum. Prog Neurobiol. 1991;36(6):437–463. doi: 10.1016/0301-0082(91)90012-p. [DOI] [PubMed] [Google Scholar]
  11. Kaneda M., Wakamori M., Ito C., Akaike N. Low-threshold calcium current in isolated Purkinje cell bodies of rat cerebellum. J Neurophysiol. 1990 May;63(5):1046–1051. doi: 10.1152/jn.1990.63.5.1046. [DOI] [PubMed] [Google Scholar]
  12. Llinás R., Sugimori M. Calcium conductances in Purkinje cell dendrites: their role in development and integration. Prog Brain Res. 1979;51:323–334. doi: 10.1016/S0079-6123(08)61312-6. [DOI] [PubMed] [Google Scholar]
  13. Llinás R., Sugimori M., Lin J. W., Cherksey B. Blocking and isolation of a calcium channel from neurons in mammals and cephalopods utilizing a toxin fraction (FTX) from funnel-web spider poison. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1689–1693. doi: 10.1073/pnas.86.5.1689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marr D. A theory of cerebellar cortex. J Physiol. 1969 Jun;202(2):437–470. doi: 10.1113/jphysiol.1969.sp008820. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Midtgaard J., Lasser-Ross N., Ross W. N. Spatial distribution of Ca2+ influx in turtle Purkinje cell dendrites in vitro: role of a transient outward current. J Neurophysiol. 1993 Dec;70(6):2455–2469. doi: 10.1152/jn.1993.70.6.2455. [DOI] [PubMed] [Google Scholar]
  16. Miyakawa H., Lev-Ram V., Lasser-Ross N., Ross W. N. Calcium transients evoked by climbing fiber and parallel fiber synaptic inputs in guinea pig cerebellar Purkinje neurons. J Neurophysiol. 1992 Oct;68(4):1178–1189. doi: 10.1152/jn.1992.68.4.1178. [DOI] [PubMed] [Google Scholar]
  17. Rall W., Burke R. E., Smith T. G., Nelson P. G., Frank K. Dendritic location of synapses and possible mechanisms for the monosynaptic EPSP in motoneurons. J Neurophysiol. 1967 Sep;30(5):1169–1193. doi: 10.1152/jn.1967.30.5.1169. [DOI] [PubMed] [Google Scholar]
  18. Regan L. J. Voltage-dependent calcium currents in Purkinje cells from rat cerebellar vermis. J Neurosci. 1991 Jul;11(7):2259–2269. doi: 10.1523/JNEUROSCI.11-07-02259.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rittenhouse A. R., Hess P. Microscopic heterogeneity in unitary N-type calcium currents in rat sympathetic neurons. J Physiol. 1994 Jan 1;474(1):87–99. doi: 10.1113/jphysiol.1994.sp020005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shambes G. M., Gibson J. M., Welker W. Fractured somatotopy in granule cell tactile areas of rat cerebellar hemispheres revealed by micromapping. Brain Behav Evol. 1978;15(2):94–140. doi: 10.1159/000123774. [DOI] [PubMed] [Google Scholar]
  21. Turner R. W., Meyers D. E., Barker J. L. Fast pre-potential generation in rat hippocampal CA1 pyramidal neurons. Neuroscience. 1993 Apr;53(4):949–959. doi: 10.1016/0306-4522(93)90480-4. [DOI] [PubMed] [Google Scholar]

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