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. 1955 Sep;34(9):1427–1436. doi: 10.1172/JCI103192

PAPER ELECTROPHORESIS AS A QUANTITATIVE METHOD: MEASUREMENT OF ALPHA AND BETA LIPOPROTEIN CHOLESTEROL

Thomas A Langan 1, E L Durrum 1,1, William P Jencks 1,2
PMCID: PMC438716  PMID: 13252090

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACKERMANN P. G., KOUNTZ W. B. Zone electrophoresis in the study of serum lipoproteins. I. Methods and preliminary results. J Lab Clin Med. 1954 Oct;44(4):517–530. [PubMed] [Google Scholar]
  2. BARR D. P., RUSS E. M., EDER H. A. Protein-lipid relationships in human plasma. II. In atherosclerosis and related conditions. Am J Med. 1951 Oct;11(4):480–493. doi: 10.1016/0002-9343(51)90183-0. [DOI] [PubMed] [Google Scholar]
  3. BOLINGER R. E., GRADY H. J., SLINKER B. J. The effect of injected heparin on the electrophoresis of the lipoproteins in patients with hypercholesterolemia. Am J Med Sci. 1954 Feb;227(2):193–200. doi: 10.1097/00000441-195402000-00012. [DOI] [PubMed] [Google Scholar]
  4. BOYD G. S. The estimation of serum lipoproteins; a micromethod based on zone electrophoresis and cholesterol estimations. Biochem J. 1954 Dec;58(4):680–685. doi: 10.1042/bj0580680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BOYLE E., BRAGDON J. H., BROWN R. K. Role of heparin in in vitro production of alpha1 lipoproteins in human plasma. Proc Soc Exp Biol Med. 1952 Nov;81(2):475–477. doi: 10.3181/00379727-81-19915. [DOI] [PubMed] [Google Scholar]
  6. DURRUM E. L., PAUL M. H., SMITH E. R. B. Lipid detection in paper electrophoresis. Science. 1952 Oct 17;116(3016):428–430. doi: 10.1126/science.116.3016.428. [DOI] [PubMed] [Google Scholar]
  7. FASOLI A. Electrophoresis of serum lipoproteins on filter-paper. Acta Med Scand. 1953;145(3):233–235. doi: 10.1111/j.0954-6820.1953.tb07014.x. [DOI] [PubMed] [Google Scholar]
  8. FURST V., Jr, LANGE R. Evaluation of an acetic acid-sulphuric acid method for quantitative determination of cholesterol in serum. Scand J Clin Lab Invest. 1954;6(1):60–64. doi: 10.1080/00365515409134834. [DOI] [PubMed] [Google Scholar]
  9. GOFMAN J. W., JONES H. B., LINDGREN F. T., LYON T. P., ELLIOTT H. A., STRISOWER B. Blood lipids and human atherosclerosis. Circulation. 1950 Aug;2(2):161–178. doi: 10.1161/01.cir.2.2.161. [DOI] [PubMed] [Google Scholar]
  10. JENCKS W. P., DURRUM E. L. Paper electrophoresis as a quantitative method: the staining of serum lipoproteins. J Clin Invest. 1955 Sep;34(9):1437–1448. doi: 10.1172/JCI103193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. JENCKS W. P., JETTON M. R., DURRUM E. L. Paper electrophoresis as a quantitative method; serum proteins. Biochem J. 1955 Jun;60(2):205–215. doi: 10.1042/bj0600205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KUNKEL H. G., SLATER R. J. Lipoprotein patterns of serum obtained by zone electrophoresis. J Clin Invest. 1952 Jul;31(7):677–684. doi: 10.1172/JCI102649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KUNKEL H. G., SLATER R. J. Zone electrophoresis in a starch supporting medium. Proc Soc Exp Biol Med. 1952 May;80(1):42–44. doi: 10.3181/00379727-80-19516. [DOI] [PubMed] [Google Scholar]
  14. LEVER W. F., GURD F. R. N., UROMA E., BROWN R. K., BARNES B. A., SCHMID K., SCHULTZ E. L. Chemical, clinical and immunological studies on the products of human plasma fractionation XL. Quantitative separation and determination of the protein components in small amounts of normal human plasma. J Clin Invest. 1951 Jan;30(1):99–111. doi: 10.1172/JCI102423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LEWIS L. A., PAGE I. H. Electrophoretic and ultracentrifugal analysis of serum lipoproteins of normal, nephrotic and hypertensive persons. Circulation. 1953 May;7(5):707–717. doi: 10.1161/01.cir.7.5.707. [DOI] [PubMed] [Google Scholar]
  16. LINDGREN F. T., ELLIOTT H. A., GOFMAN J. W. The ultracentrifugal characterization and isolation of human blood lipids and lipoproteins, with applications to the study of atherosclerosis. J Phys Colloid Chem. 1951 Jan;55(1):80–93. doi: 10.1021/j150484a010. [DOI] [PubMed] [Google Scholar]
  17. ROSENBERG I. N., YOUNG E., PROGER S. Serum lipoproteins of normal and atherosclerotic persons studies by paper electrophoresis. Am J Med. 1954 Jun;16(6):818–821. doi: 10.1016/0002-9343(54)90446-5. [DOI] [PubMed] [Google Scholar]
  18. RUSS E. M., EDER H. A., BARR D. P. Protein-lipid relationships in human plasma. I. In normal individuals. Am J Med. 1951 Oct;11(4):468–479. doi: 10.1016/0002-9343(51)90182-9. [DOI] [PubMed] [Google Scholar]
  19. SOMMERFELT S. C. Paper electrophoresis of isolated plasma protein fractions. Scand J Clin Lab Invest. 1953;5(4):299–307. doi: 10.3109/00365515309094203. [DOI] [PubMed] [Google Scholar]
  20. SPERRY W. M., WEBB M. A revision of the Schoenheimer-Sperry method for cholesterol determination. J Biol Chem. 1950 Nov;187(1):97–106. [PubMed] [Google Scholar]
  21. WILLIAMS F. G., Jr, PICKELS E. G., DURRUM E. L. Improved hanging-strip paper-electrophoresis technique. Science. 1955 Jun 10;121(3154):829–830. doi: 10.1126/science.121.3154.829. [DOI] [PubMed] [Google Scholar]
  22. WUNDERLY C., PILLER S. Die Färbung der im Blutserum enthaltenen Proteine, Lipoide und Kohlenhydrate nach papier-elektrophorese; eine chemische trias. Klin Wochenschr. 1954 May 1;32(17-18):425–432. doi: 10.1007/BF01468961. [DOI] [PubMed] [Google Scholar]
  23. ZAK B., DICKENMAN R. C., WHITE E. G., BURNETT H., CHERNEY P. J. Rapid estimation of free and total cholesterol. Am J Clin Pathol. 1954 Nov;24(11):1307–1315. doi: 10.1093/ajcp/24.11_ts.1307. [DOI] [PubMed] [Google Scholar]
  24. ZLATKIS A., ZAK B., BOYLE A. J. A new method for the direct determination of serum cholesterol. J Lab Clin Med. 1953 Mar;41(3):486–492. [PubMed] [Google Scholar]

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