Figure 4.

Transcription of DDT factors is not dramatically altered in esa1-L254P mutants upon MMS treatment. (A) Viability assays as described in Figure 1 were performed for wild type (JC470), rev3Δ (JC2289), ubc13Δ (JC2291), hhf2^K→R (JC3178), hhf2^K→R/rev3Δ (JC3195), hhf2^K→R/ubc13Δ (JC3179) and (B) htz1Δ (JC2090), htz1Δ/rev3Δ (JC2762), and htz1Δ/ubc13Δ (JC2764). (C–G) qRT-PCR as described in Materials and Methods was performed on wild-type and esa1-L254P cells treated with α-factor for 2 hr followed by release into normal YPAD (S phase) or YPAD with 0.05% MMS (S + MMS) for 1 hr. Candidate genes in the DDT pathway were analyzed for expression with and without MMS. The qRT-PCR values from target genes were normalized to ALG9 (Table S1), as its transcript levels were most stable throughout the cell cycle.