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. 2015 Apr 2;4:161. doi: 10.1186/s40064-015-0932-8

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© Donis-Maturano et al.; licensee Springer. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Kinetics of apoptosis of macrophages and dendritic cells upon exposure to NETs. Percentage of macrophages (A,C) or dendritic cells (B,D) incubated only in medium, with NETs, or NETs sn, and labeled for active Caspase-3 (A,B) and AIF (C,D). Active Caspase-3 and AIF were evaluated at 0.5, 3, 6, 12 and 24 h post-stimulation with NETs. Positive controls represent Mfs or DCs cultured either in PBS (for Caspase-3) or with CCCP (for AIF) for 24 hours before analysis. Mfs: Macrophages, DC: Dendritic cells, NETs sn: NETs supernatants, CCCP: Carbonyl cyanide m-chlorophenyl hydrazone. *P = 0.01; **P = 0.001; ***P < 0.0001, one-way ANOVA.