Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 15;142(8):1528–1541. doi: 10.1242/dev.119271

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 2. — WNT3A, BMP4 and RA are key regulators of LM differentiation to epicardium. (A) Expression of epicardial genes by qRT-PCR in epicardium-like cells generated from LM after 10 days of treatment with different combinations of FGF2, BMP4, WNT3A and retinoic acid (RA). Significant differences compared with LM are indicated in black, whereas intergroup differences are indicated in red. ***P<0.001, **P<0.01, *P<0.05. (B) Temporal expression of epicardial genes in BMP4+RA- and WNT3A+BMP4+RA-treated cells determined by qRT-PCR. ***P<0.001, *P<0.05. (C) Percentage of WT1⁺ cells in BMP4+RA and WNT3A+BMP4+RA-treated day 10 epicardial cells. H9-derived neuroectoderm (NE) served as negative control. (D) Inhibition of endogenous WNT signalling by differentiating LM with IWP2 resulted in reduced expression of epicardial genes (WT1 and TCF21). **P<0.01, *P<0.05.