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. 2015 Apr 15;142(8):1528–1541. doi: 10.1242/dev.119271

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Epicardium-derived cardiac fibroblast differentiation. (A) Analysis of fibroblast (PDGFRA and POSTN) and endothelial (NOS3 and PECAM1) markers in epicardium-derived cells (EPDCs) obtained with VEGF and FGF treatment. ***P<0.001. (B) Percentage of POSTN⁺ cells in epicardium-derived cardiac fibroblasts (EPI-CFs) determined by flow cytometry. Rabbit IgG isotype and HUVECs were used as negative controls. (C) The majority of EPI-CFs immunostained positive for DDR2, whereas HUVECs displayed negligible expression. Scale bars: 100 μm.