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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Hypertension. 2015 Mar 23;65(5):1118–1125. doi: 10.1161/HYPERTENSIONAHA.114.05096

Figure 3.

Figure 3

Effect of miR-217 on the expression of TGFβ1 and EMT markers in human renal proximal tubule cells (RPTCs) from subjects carrying D2R-WT or D2R-SNPs.

A-D. mRNA expressions of TGFβ1, MMP3, FN1,and Col1a in RPTC-D2R-WT or RPTC-D2R-SNPs before and after transfection of miR-217 mimic or inhibitor were quantified by qRT-PCR as above, n=4-5/group. *P<0.05 vs control; ANOVA followed by Holm-Sidak test.

E. Protein expression of TGFβ1 in RPTC-D2R-WT or RPTC-D2R-SNPs treated as above of the data. n=4-5/group. *P<0.05 vs control; ANOVA followed by Holm-Sidak test

F. Laser-scanning confocal images of immunofluorescence staining of TGFβ1 in RPTC-D2R-WT treated with control or miR-217 inhibitor and in RPTC-D2R-SNPs treated with control or miR-217 mimic. DAPI was used to immunostain the nuclei.