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. 1994 May 24;91(11):5080–5084. doi: 10.1073/pnas.91.11.5080

Nuclear transformation of Volvox carteri.

B Schiedlmeier 1, R Schmitt 1, W Müller 1, M M Kirk 1, H Gruber 1, W Mages 1, D L Kirk 1
PMCID: PMC43935  PMID: 8197189

Abstract

Stable nuclear transformation of Volvox carteri was achieved using the cloned V. carteri nitA+ gene (which encodes nitrate reductase) to complement a nitA- mutation. Following bombardment of mutant cells with plasmid-coated gold particles, putative transformants able to utilize nitrate as a nitrogen source were recovered with an efficiency of approximately 2.5 x 10(5). DNA analysis indicated that the plasmid integrated into the genome, often in multiple copies, at sites other than the nitA locus. Cotransformants were recovered with a frequency of 40-80% when cells were cobombarded with a selected and an unselected marker. Thus, V. carteri becomes one of the simplest multicellular organisms that is accessible to detailed molecular studies of genes regulating cellular differentiation and morphogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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