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. Author manuscript; available in PMC: 2016 Apr 9.
Published in final edited form as: Cell. 2015 Apr 9;161(2):264–276. doi: 10.1016/j.cell.2015.02.047

Figure 2. Indigenous Spore-forming Bacteria Increase 5-HT Levels in Colon Enterochromaffin Cells.

Figure 2

(A) Representative images of colons stained for chromagranin A (CgA) (left), 5-HT (center) and merged (right). Arrows indicate CgA-positive cells that lack 5-HT staining. n=3–7 mice/group.

(B) Quantitation of 5-HT+ cell number per area of colonic epithelial tissue. n=3–7 mice/group.

(C) Quantitation of CgA+ cell number per area of colonic epithelial tissue. n=3–7 mice/group.

(D) Ratio of 5-HT+ cells/CgA+ cells per area of colonic epithelial tissue. n=3–7 mice/group.

Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. SPF=specific pathogen-free (conventionally-colonized), GF=germ-free, CONV.=SPF conventionalized, ABX=antibiotic-treated, Sp=spore-forming bacteria, PCPA=para-chlorophenylalanine. See also Figure S2.