Table 2.
Identification of proteins differentially expressed after treatment with 10μM of DETA/NO in absence of serum in Human Pancreatic Islets
| Spot ID | Official symbol | Name | Protein MW/PtdIns | No. peptides identified MASCOT | Mascot Protein Score | Biological Process |
|---|---|---|---|---|---|---|
| 1 | POLR3E | Polymerase (RNA) III (DNA directed) polypeptide E (80kD) | 99101.4 /9.47 | 11 | 50 * | Cell Death and Survival |
| 2 | GANAB | Glucosidase, α; neutral AB | 107262.8/ 5.74 | 28 | 578 | Metabolism |
| 3 | VCP | Vasolin containing protein | 84954.4 /5.16 | 32 | 390 | Cell Death and Survival |
| 4 | HSP90AA1 | Heat shock protein 90kDa α (cytosolic), class A member 1 | 80579.1/ 5.26 | 26 | 800 | Cell Death and Survival |
| 5 | HSPA5 | Heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) | 68354.4 /5.15 | 31 | 1060 | aCell Death and Survival |
| 6 | HSPA9 | Heat shock 70kDa protein 9 (mortalin) | 73929.9 /5.81 | 18 | 582 | aCell Death and Survival |
| 7 | HSPA8 | Heat shock 70kDa protein 8 | 70989.2 /5.24 | 22 | 837 | Cell Death and Survival |
| 8 | PRSS1 | Protease, serine, 1 (trypsin 1) | 9219 /10.01 | 3 | 37 * | Metabolism |
| 9 | KRT1 | Keratin 1 | 66149/ 8.16 | 13 | 76 | Cell Proliferation |
| 10 | P4HB | Prolyl 4-hydroxylase, β polypeptide | 57453.7 /4.73 | 28 | 875 | Cell Proliferation |
| 11 | TUBA1B | Tubulin α-1B | 46781/ 4.96 | 19 | 648 | Cell Proliferation |
| 12 | PDIA3 | Protein disulfide isomerase family A, member 3 | 54541.4 /5.61 | 22 | 407 | Cell Death and Survival |
| 13 | POLR3E | Polymerase (RNA) III (DNA directed) polypeptide E (80kD) | 99101.4 /9.47 | 10 | 48 * | Cell Death and Survival |
| 14 | TUBB | Tubulin, β class I | 48319.4 /5.17 | 26 | 772 | Cell Proliferation |
| 15 | ALDH1A1 | Aldehyde dehydrogenase 1 | 51742.4 /6.68 | 17 | 671 | Metabolism |
| 16 | LUZP2 | Leucine zipper protein 2 | 10021.2 /7.94 | 8 | 75 | Development |
| 17 | ALDH1A1 | Aldehyde dehydrogenase 1 family, member A1 | 51742.4 /6.68 | 17 | 728 | Metabolism |
| 18 | HNRNPH1 | Heterogeneous nuclear ribonucleoprotein H1 (H) | 43790.1 /6.29 | 7 | 98 | Cell Death and Survival |
| 19 | ATP5A1 | ATP synthase, H+ transporting, mitochondrial F1 complex, α subunit 1, cardiac muscle | 59827.6 /9.16 | 27 | 735 | Cell Death and Survival |
| 20 | PDIA6 | Protein disulfide isomerase family A, member 6 | 46512.3 /4.95 | 14 | 907 | aCell Death and Survival |
| 21 | DNAJC19 | DnaJ (Hsp40) homolog, subfamily C, member 19 | 17412,9/9,71 | 6 | 38 * | Cell Death and Survival |
| 22 | ENO1 | Enolase 1, (α) | 42720.9 /6.97 | 25 | 673 | Metabolism |
| 23 | TBX3 | T-box 3 | 57754.1 /6.95 | 9 | 84 | Development |
| 24 | ACTG1 | Actin, gamma 1 | 41334.6 /5.56 | 15 | 636 | Cell Proliferation |
| 25 | KRT10 | Keratin 10 | 56862.9/ 5.05 | 11 | 81 | Cell Death and Survival |
| 26 | YWHAE | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon | 26911.5 /4.92 | 16 | 431 | Cell Death and Survival |
| 27 | SCGN | Secretagogin, EF-hand calcium binding protein | 32190.2 /5.25 | 23 | 603 | Cell Proliferation |
| 28 | TPI1 | Triosephosphate isomerase 1 | 26937.8 /6.45 | 20 | 774 | Metabolism |
| 29 | PRDX2 | Peroxiredoxin 2 | 16165.3 /5.71 | 8 | 343 | aCell Death and Survival |
*
Protein score is −10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 71 are significant (P < 0.05).
a
Involved in β-cell survival