An error was introduced during the production process. A fixed-width font should have been used for the sequences in Table 3. The publisher apologizes for the error.
Table 3. Binding of designed peptides to their bZIP targets.
| Design | Target | K d at 37 °C (nM) | Designed sequence |
|---|---|---|---|
| XBP1-d1 1 | XBP1 | 172 ± 65 | EREAQLENRVAHLKEKNQELKAQNLHLKEALSEAQNRNQELKNDA |
| XBP1-d2 | XBP1 | ≥ 5,000 | AETDQLENRVKDLKKKNESLKEEKRQASNKYKALLTNNRSLKVKA |
| XBP1-d2* 2 | XBP1 | ≥ 5,000 (269 at 23 °C) | AETDQLENRVKDLKKKNESLKEEKRQAKNKLKALLTNNRSLKVKA |
| JUN-d1 1 | JUN | 5.8 ± 0.7 | SIAATLEKEEANLEKMNKKLAAEIESLLKEKDKLESVLNYHE |
| JUN-d2 | JUN | 1 | QRALQLQKEKERLEKMNKKLAAEIESLLEERERLESVLNYHE |
| ATF3-d1 | ATF3 | ~1,000 | NDLARLENKAEELKVQNRILVDERKYLQREISELHDELAAHE |
| ATF3-d2 | ATF3 | 564 | NLVAQLEKKNEALKAENAALEIERIQLQDKIEELKYELAAIE |
| ATF3-d3 | ATF3 | ≥ 5,000 (117 at 4 °C) | KDAASLENKKEELKVQNRILVDERKYLQMMNSELKDELAAHE |
| ATF4-d1 1 | ATF4 | 9.3 ± 2.3 | NQIKTLRTRLSKLRKDNLQLEKDIANLERKAKDLRAEKEQLEYEL |
| ATF5-d1 1 | ATF5 | 1680 ± 1050 | KRIAYLRQRIAELRNENHVLESRIQRMEKEKDALQQDRDHLEYEL |
| ATF5-d1 3 | ATF4 | 4.9 ± 1.0 | (same as above) |
1 These four designs were chosen for further characterization.
2 A double mutant of XBP1-d2, in which serine at position 4e and tyrosine at position 5a were mutated to lysine and leucine, respectively.
3 Although ATF5 was used in the computational design, binding was tighter to ATF4, which shares 69.2% sequence identity with ATF5 in a, d, e and g positions.
Please view the corrected table here:
Reference
- 1. Potapov V, Kaplan JB, Keating AE (2015) Data-Driven Prediction and Design of bZIP Coiled-Coil Interactions. PLoS Comput Biol 11(2): e1004046 doi: 10.1371/journal.pcbi.1004046 [DOI] [PMC free article] [PubMed] [Google Scholar]