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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: Nature. 2015 Mar 25;520(7546):248–252. doi: 10.1038/nature14337

Figure 1. siRNA-directed de novo formation of heterochromatin.

Figure 1

a, ade6-hp RNA producing locus and siRNA target in trans in the sms0 strain. b, Silencing assay performed with freshly generated Paf1C mutants. c, Silencing assay performed with red colonies from b. d, ade6+ siRNAs direct the methylation of H3K9 in trans at the trp1+::ade6+ locus in Paf1C mutant cells. Error bars, SEM; n=3 technical replicates. e, Gene silencing at the trp1+::ade6+ locus depends on the same factors as constitutive heterochromatin at centromeric repeats.