Table 1.
Antibody Information
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised (Monoclonal or Polyclonal) | Dilution Used |
|---|---|---|---|---|---|
| IBA1 | 17-kDa EF hand protein | Anti-Iba1 | Wago; 019-19741 | Rabbit, polyclonal | 1:200 |
| AgRP | Synthetic peptide within mouse AGRP (C terminal) | Anti-AgRP | Abcam; ab181493 | Guinea pig, polyclonal | 1:500 |
| POMC | C-terminal amino acids 256–267 of human POMC | Anti-POMC | Abcam; ab32893 | Goat, polyclonal | 1:300 |
| IBA1 | Amino acids 135–147 of human Iba1 | Anti-Iba1 | Abcam; ab5076 | Goat, polyclonal | 1:1000 |
| TLR4 | Synthetic peptide corresponding to human TLR4 amino acids 420–435 | Anti-TLR4 | Abcam, ab13556 | Rabbit, polyclonal | 1:200 |
| TLR4 | Clone MTS510 | MAb mTLR4/MD2 | Invivogen; mab-mtlr4md2 | Mouse, monoclonal | 0.1 μg/mL |