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. 1980 Jan;65(1):27–32. doi: 10.1104/pp.65.1.27

Some New Aspects of the in Vivo Assay for Nitrate Reductase in Wheat (Triticum aestivum L.) Leaves

I. REEVALUATION OF NITRATE POOL SIZES 1

Richard H Hageman 1, Andrew J Reed 1, Rise A Femmer 1, Joseph H Sherrard 1, Michael J Dalling 1,2
PMCID: PMC440260  PMID: 16661137

Abstract

Experiments were carried out to clarify problems encountered in measuring metabolic and storage pool sizes of nitrate in wheat leaf sections with an in vivo nitrate reductase assay. The leaf sections were from seedlings grown on 15 millimolar nitrate. Data obtained show that the cessation of nitrite accumulation, used as an index of the active nitrate pool size, could be caused by lack of anaerobiosis in the assay system, the lack of energy for nitrate reduction, or a loss of nitrate reductase activity. Availability of nitrate was never the limiting factor in this system. It is concluded that pool sizes of nitrate cannot be determined in wheat leaves with the in vivo assays employed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aslam M., Huffaker R. C., Rains D. W., Rao K. P. Influence of light and ambient carbon dioxide concentration on nitrate assimilation by intact barley seedlings. Plant Physiol. 1979 Jun;63(6):1205–1209. doi: 10.1104/pp.63.6.1205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aslam M., Oaks A. Effect of glucose on the induction of nitrate reductase in corn roots. Plant Physiol. 1975 Nov;56(5):634–639. doi: 10.1104/pp.56.5.634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aslam M., Oaks A., Huffaker R. C. Effect of light and glucose on the induction of nitrate reductase and on the distribution of nitrate in etiolated barley leaves. Plant Physiol. 1976 Oct;58(4):588–591. doi: 10.1104/pp.58.4.588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ferrari T. E., Yoder O. C., Filner P. Anaerobic nitrite production by plant cells and tissues: evidence for two nitrate pools. Plant Physiol. 1973 Mar;51(3):423–431. doi: 10.1104/pp.51.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klepper L., Flesher D., Hageman R. H. Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. Plant Physiol. 1971 Nov;48(5):580–590. doi: 10.1104/pp.48.5.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Neyra C. A., Hageman R. H. Relationships between Carbon Dioxide, Malate, and Nitrate Accumulation and Reduction in Corn (Zea mays L.) Seedlings. Plant Physiol. 1976 Dec;58(6):726–730. doi: 10.1104/pp.58.6.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nicholas J. C., Harper J. E., Hageman R. H. Nitrate Reductase Activity in Soybeans (Glycine max [L.] Merr.): II. Energy Limitations. Plant Physiol. 1976 Dec;58(6):736–739. doi: 10.1104/pp.58.6.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ohmura T., Howell R. W. Inhibitory Effect of Water on Oxygen Consumption by Plant Materials. Plant Physiol. 1960 Mar;35(2):184–188. doi: 10.1104/pp.35.2.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Scholl R. L., Harper J. E., Hageman R. H. Improvements of the nitrite color development in assays of nitrate reductase by phenazine methosulfate and zinc acetate. Plant Physiol. 1974 Jun;53(6):825–828. doi: 10.1104/pp.53.6.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sherrard J. H., Green D. L., Swinden L. B., Dalling M. J. Identification of wheat (Triticum aestivum L.) chromosomes with genes controlling the level of nitrate reductase, nitrite reductase, and acid proteinase using the Chinese Spring-Hope substitution lines. Biochem Genet. 1976 Dec;14(11-12):905–912. doi: 10.1007/BF00485123. [DOI] [PubMed] [Google Scholar]

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