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. 1980 Jan;65(1):85–87. doi: 10.1104/pp.65.1.85

Assessment of Cytoplasmic Contaminations in Isolated Vacuole Preparations 1,2

Arie Admon 1,2, Benjamin Jacoby 1,2, Eliezer E Goldschmidt 1,2
PMCID: PMC440272  PMID: 16661150

Abstract

A method for testing the purity of isolated vacuoles is proposed. Preparations of isolated vacuoles often contain “impure vacuoles,” namely, vacuoles contaminated with some cytoplasmic remnants. Such impure vacuoles are stained by the vital fluorescent dye fluorescein-diacetate. Vacuoles enclosed only by the tonoplast, “naked vacuoles,” do not stain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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