Abstract
We have studied the biological effects of two structural analogs of cytokinins, 3-methyl-7-(pentylamino)pyrazolo(4,3-d)pyrimidine and 3-methyl-7-(benzylamino)pyrazolo(4,3-d)pyrimidine, on tobacco cell suspension cultures. These two cytokinin analogs are highly inhibitory to cytokinin-autonomous and cytokinin-requiring tobacco cells. The growth inhibitory effect is markedly antagonized by benzyladenine, but not by adenine. Cell suspensions of tobacco cells of a cytokinin-autonomous strain become cytokinin-dependent in the presence of 0.1 micromolar 3-methyl-7-(benzylamino)pyrazolo(4,3-d)pyrimidine. It is also demonstrated that growth inhibition of cell suspension cultures is accompanied by cell death and that only dividing cells are sensitive to this cytotoxic effect.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hecht S. M., Bock R. M., Schmitz R. Y., Skoog F., Leonard N. J. Cytokinins: development of a potent antagonist. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2608–2610. doi: 10.1073/pnas.68.10.2608. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hecht S. M., Frye R. B., Werner D., Hawrelak S. D., Skoog F., Schmitz R. Y. On the "activation" of cytokinins. J Biol Chem. 1975 Sep 25;250(18):7343–7351. [PubMed] [Google Scholar]