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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1994 Jun 7;91(12):5552–5556. doi: 10.1073/pnas.91.12.5552

Selective inactivation of viruses in the presence of human platelets: UV sensitization with psoralen derivatives.

R P Goodrich 1, N R Yerram 1, B H Tay-Goodrich 1, P Forster 1, M S Platz 1, C Kasturi 1, S C Park 1, N J Aebischer 1, S Rai 1, L Kulaga 1
PMCID: PMC44034  PMID: 8202526

Abstract

Inactivation of viruses in blood products requires that the method employed display selectivity in its action for viral elements while not affecting the biological entity of interest. Several methods have been developed for the treatment of human plasma or products derived from human plasma. An effective technique for the treatment of the cellular components of blood has been lacking, in part due to the inability to develop agents capable of selectively targeting viral agents in the milieu of cellular material. In this paper, we examine the behavior of a group of viral sensitizers designed to be added to cellular samples and be activated upon exposure to UVA light. Upon activation, these agents are capable of disrupting nucleic acids of the virus in a manner that renders them inactive for proliferation. The selectivity observed in this inactivation is determined by the chemical structure of the sensitizer, which can be varied to increase viral killing capacity while diminishing collateral damage to cellular and protein constituents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armitage W. J., Parmar N., Hunt C. J. The effects of osmotic stress on human platelets. J Cell Physiol. 1985 May;123(2):241–248. doi: 10.1002/jcp.1041230214. [DOI] [PubMed] [Google Scholar]
  2. Isaacs S. T., Shen C. K., Hearst J. E., Rapoport H. Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA. Biochemistry. 1977 Mar 22;16(6):1058–1064. doi: 10.1021/bi00625a005. [DOI] [PubMed] [Google Scholar]
  3. Kunicki T. J., Tuccelli M., Becker G. A., Aster R. H. A study of variables affecting the quality of platelets stored at "room temperature". Transfusion. 1975 Sep-Oct;15(5):414–421. doi: 10.1046/j.1537-2995.1975.15576082215.x. [DOI] [PubMed] [Google Scholar]
  4. Margolis-Nunno H., Williams B., Rywkin S., Geacintov N., Horowitz B. Virus sterilization in platelet concentrates with psoralen and ultraviolet A light in the presence of quenchers. Transfusion. 1992 Jul-Aug;32(6):541–547. doi: 10.1046/j.1537-2995.1992.32692367198.x. [DOI] [PubMed] [Google Scholar]
  5. O'Brien J. M., Gaffney D. K., Wang T. P., Sieber F. Merocyanine 540-sensitized photoinactivation of enveloped viruses in blood products: site and mechanism of phototoxicity. Blood. 1992 Jul 1;80(1):277–285. [PubMed] [Google Scholar]
  6. Prodouz K. N., Lytle C. D., Keville E. A., Budacz A. P., Vargo S., Fratantoni J. C. Inhibition by albumin of merocyanine 540-mediated photosensitization of platelets and viruses. Transfusion. 1991 Jun;31(5):415–422. doi: 10.1046/j.1537-2995.1991.31591263196.x. [DOI] [PubMed] [Google Scholar]
  7. Rai S., Kasturi C., Grayzar J., Platz M. S., Goodrich R. P., Yerram N. R., Wong V., Tay-Goodrich B. H. Dramatic improvements in viral inactivation with brominated psoralens, naphthalenes and anthracenes. Photochem Photobiol. 1993 Jul;58(1):59–65. doi: 10.1111/j.1751-1097.1993.tb04904.x. [DOI] [PubMed] [Google Scholar]
  8. Wagner S. J., Friedman L. I., Dodd R. Y. Approaches to the reduction of viral infectivity in cellular blood components and single donor plasma. Transfus Med Rev. 1991 Jan;5(1):18–32. doi: 10.1016/s0887-7963(91)70190-9. [DOI] [PubMed] [Google Scholar]

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