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. 1980 Mar;65(3):451–454. doi: 10.1104/pp.65.3.451

Photoactivation of Chlorophyll Synthesis and Cytochrome Oxidase Activity in Anaerobically Germinated Seedlings of Echinochloa crusgalli var. Oryzicola

Wei-Yeh Wang 1
PMCID: PMC440352  PMID: 16661211

Abstract

When seeds of Echinochloa crusgalli var. oryzicola are germinated in dark anaerobic conditions (99.995% N2), the seedlings do not have detectable protochlorophyll(ide). Two hours after exposure to light aerobic conditions, they begin to synthesize chlorophyll. The lag in greening is shorter in seedlings exposed to light for 24 hours before exposure to air. Seedlings maintained in light anaerobic conditions exhibit no lag in greening upon transfer to an aerobic environment. Preillumination of anaerobically grown seedlings does not result in any chlorophyll accumulation. Phytochrome is probably the receptor for photoactivation of chlorophyll synthesis, since activation is achieved by red light alone, but not by far red light or red plus far red light. The cytochrome oxidase activity in anaerobically germinated seedlings is 30% of the normal level found in aerobically grown seedlings. Preillumination was also found to activate the ability of anaerobically germinated seedlings to increase their cytochrome oxidase activity upon exposure to air.

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Selected References

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  1. Barrett J. The incorporation of [2-14C]glycine and delta-amino[4-14C]-laevulinic acid into the prosthetic groups of cytochrome oxidase and other cytochromes in yeast adapting to oxygen. Biochim Biophys Acta. 1969 May 6;177(3):442–455. doi: 10.1016/0304-4165(69)90307-9. [DOI] [PubMed] [Google Scholar]
  2. Beale S. I., Castelfranco P. A. The Biosynthesis of delta-Aminolevulinic Acid in Higher Plants: II. Formation of C-delta-Aminolevulinic Acid from Labeled Precursors in Greening Plant Tissues. Plant Physiol. 1974 Feb;53(2):297–303. doi: 10.1104/pp.53.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Castelfranco P. A., Rich P. M., Beale S. I. The Abolition of the Lag Phase in Greening Cucumber Cotyledons by Exogenous delta-Aminolevulinic Acid. Plant Physiol. 1974 Apr;53(4):615–618. doi: 10.1104/pp.53.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gough S. Defective synthesis of porphyrins in barley plastids caused by mutation in nuclear genes. Biochim Biophys Acta. 1972 Nov 24;286(1):36–54. doi: 10.1016/0304-4165(72)90086-4. [DOI] [PubMed] [Google Scholar]
  5. Kasemir H., Oberdorfer U., Mohr H. A twofold action of phytochrome in controlling chlorophyll a accumulation. Photochem Photobiol. 1973 Dec;18(6):481–486. doi: 10.1111/j.1751-1097.1973.tb06453.x. [DOI] [PubMed] [Google Scholar]
  6. Klein S., Katz E., Neeman E. Induction of delta-Aminolevulinic Acid Formation in Etiolated Maize Leaves Controlled by Two Light Systems. Plant Physiol. 1977 Sep;60(3):335–338. doi: 10.1104/pp.60.3.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klein S., Schiff J. A., Holowinsky A. W. Events surrounding the early development of Euglena chloroplasts. II. Normal development of fine structure and the consequences of preillumination. Dev Biol. 1972 May;28(1):253–273. doi: 10.1016/0012-1606(72)90142-x. [DOI] [PubMed] [Google Scholar]
  8. Klein S., Schiff J. A. The Correlated Appearance of Prolamellar Bodies, Protochlorophyll(ide) Species, and the Shibata Shift during Development of Bean Etioplasts in the Dark. Plant Physiol. 1972 Apr;49(4):619–626. doi: 10.1104/pp.49.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Price L., Klein W. H. Red, far-red response & chlorophyll synthesis. Plant Physiol. 1961 Nov;36(6):733–735. doi: 10.1104/pp.36.6.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schiff J. A. A green safelight for the study of chloroplast development and other photomorphogenetic. Methods Enzymol. 1972;24:321–322. doi: 10.1016/0076-6879(72)24079-4. [DOI] [PubMed] [Google Scholar]
  11. Wang W. Y. Genetic control of chlorophyll biosynthesis in Chlamydomonas reinhardtii. Int Rev Cytol Suppl. 1978;Suppl 8:335–354. doi: 10.1016/s0074-7696(08)60477-5. [DOI] [PubMed] [Google Scholar]
  12. Wang W. Y., Wang W. L., Boynton J. E., Gillham N. W. Genetic control of chlorophyll biosynthesis in Chlamydomonas. Analysis of mutants at two loci mediating the conversion of protoporphyrin-IX to magnesium protoporphyrin. J Cell Biol. 1974 Dec;63(3):806–823. doi: 10.1083/jcb.63.3.806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yonetani T. Studies on cytochrome c peroxidase. II. Stoichiometry between enzyme, H2O2, and ferrocytochrome c and enzymic determination of extinction coefficients of cytochrome c. J Biol Chem. 1965 Nov;240(11):4509–4514. [PubMed] [Google Scholar]

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