Abstract
During photosynthesis by mesophyll protoplasts of wheat and tobacco, a linear efflux of sucrose and hexoses to the medium was observed, with the size of the intraprotoplast sugar pools remaining constant. Efflux of metabolites labeled by 14CO2 fixation was initially low because of dilution by internal pools, but increased exponentially with time. The results have significance both in terms of the mechanism of translocation and the use of isolated protoplasts in photosynthetic studies.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cataldo D. A. Vein Loading: The Role of the Symplast in Intercellular Transport of Carbohydrate between the Mesophyll and Minor Veins of Tobacco Leaves. Plant Physiol. 1974 Jun;53(6):912–917. doi: 10.1104/pp.53.6.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edwards G. E., Robinson S. P., Tyler N. J., Walker D. A. Photosynthesis by isolated protoplasts, protoplast extracts, and chloroplasts of wheat: influence of orthophosphate, pyrophosphate, and adenylates. Plant Physiol. 1978 Aug;62(2):313–319. doi: 10.1104/pp.62.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geiger D. R., Sovonick S. A., Shock T. L., Fellows R. J. Role of free space in translocation in sugar beet. Plant Physiol. 1974 Dec;54(6):892–898. doi: 10.1104/pp.54.6.892. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Giaquinta R. Evidence for Phloem loading from the apoplast: chemical modification of membrane sulfhydryl groups. Plant Physiol. 1976 Jun;57(6):872–875. doi: 10.1104/pp.57.6.872. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Giaquinta R. Phloem Loading of Sucrose: pH Dependence and Selectivity. Plant Physiol. 1977 Apr;59(4):750–755. doi: 10.1104/pp.59.4.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones M. G., Outlaw W. H., Lowry O. H. Enzymic assay of 10 to 10 moles of sucrose in plant tissues. Plant Physiol. 1977 Sep;60(3):379–383. doi: 10.1104/pp.60.3.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kanai R., Edwards G. E. Purification of enzymatically isolated mesophyll protoplasts from c(3), c(4), and crassulacean Acid metabolism plants using an aqueous dextran-polyethylene glycol two-phase system. Plant Physiol. 1973 Nov;52(5):484–490. doi: 10.1104/pp.52.5.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rehfeld D. W., Jensen R. G. Metabolism of Separated Leaf Cells: III. Effects of Calcium and Ammonium on Product Distribution During Photosynthesis with Cotton Cells. Plant Physiol. 1973 Jul;52(1):17–22. doi: 10.1104/pp.52.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ziegler H. Biochemical aspects of phloem transport. Symp Soc Exp Biol. 1974;(28):43–62. [PubMed] [Google Scholar]
