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. 1980 Apr;65(4):574–577. doi: 10.1104/pp.65.4.574

Balance between Metabolite Accumulation and Transport in Relation to Photosynthesis by Isolated Spinach Chloroplasts 1

U Ingo Flügge 1,2, Martha Freisl 1, Hans W Heldt 1,2
PMCID: PMC440384  PMID: 16661240

Abstract

The relationship between the rate of orthophosphate (Pi) transport into the stroma and the rate of CO2 fixation by intact chloroplasts was investigated. High Pi concentrations in the medium lead to a depletion of stromal metabolites, due to excessive Pi transport into the stroma, resulting in the inhibition of CO2 fixation. This inhibitory effect of Pi is released by inhibitors of Pi transport, such as pyrophosphate, citrate or pyridoxal-5-phosphate. The latter compound appeared to be specially valuable in inhibiting Pi transport without affecting stromal reactions.

The Pi optima of CO2 fixation were studied when the rate of CO2 fixation or the rate of Pi transport was varied by the application of specific inhibitors. For optimal performance the rates of CO2 fixation and Pi transport have to be matched in such a way, that transport neither limits nor exceeds CO2 fixation. This accounts for the large variation in the Pi optima for CO2 fixation by different chloroplast preparations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bamberger E. S., Avron M. Site of action of inhibitors of carbon dioxide assimilation by whole lettuce chloroplasts. Plant Physiol. 1975 Oct;56(4):481–485. doi: 10.1104/pp.56.4.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassham J. A., Kirk M., Jensen R. G. Photosynthesis by isolated chloroplasts. I. Diffusion of labeled photosynthetic intermediates between isolated chloroplasts and suspending medium. Biochim Biophys Acta. 1968 Jan 15;153(1):211–218. doi: 10.1016/0005-2728(68)90162-x. [DOI] [PubMed] [Google Scholar]
  3. Fliege R., Flügge U. I., Werdan K., Heldt H. W. Specific transport of inorganic phosphate, 3-phosphoglycerate and triosephosphates across the inner membrane of the envelope in spinach chloroplasts. Biochim Biophys Acta. 1978 May 10;502(2):232–247. doi: 10.1016/0005-2728(78)90045-2. [DOI] [PubMed] [Google Scholar]
  4. Lilley R. M., Chon C. J., Mosbach A., Heldt H. W. The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitro. Biochim Biophys Acta. 1977 May 11;460(2):259–272. doi: 10.1016/0005-2728(77)90212-2. [DOI] [PubMed] [Google Scholar]
  5. Lilley R. M., Walker D. A. The reduction of 3-phosphoglycerate by reconstituted chloroplasts and by chloroplast extracts. Biochim Biophys Acta. 1974 Dec 19;368(3):269–278. doi: 10.1016/0005-2728(74)90174-1. [DOI] [PubMed] [Google Scholar]
  6. Robinson S. P., Wiskich J. T. p-Chloromercuriphenyl sulphonic acid as a specific inhibitor of the phosphate transporter in isolated chloroplasts. FEBS Lett. 1977 Jun 15;78(2):203–206. doi: 10.1016/0014-5793(77)80306-2. [DOI] [PubMed] [Google Scholar]
  7. Stokes D. M., Walker D. A. Photosynthesis by isolated chloroplasts. Inhibition by DL-glyceraldehyde of carbon dioxide assimilation. Biochem J. 1972 Aug;128(5):1147–1157. doi: 10.1042/bj1281147. [DOI] [PMC free article] [PubMed] [Google Scholar]

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