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. 1980 May;65(5):834–837. doi: 10.1104/pp.65.5.834

Partial Purification and Characterization of the mRNA for α-Amylase from Barley Aleurone Layers 1

Thomas J Mozer 1,2
PMCID: PMC440433  PMID: 16661291

Abstract

The poly(A)-containing mRNA from barley aleurone layers pretreated with gibberellic acid has been purified by phenol-chloroform extraction and repeated oligo[d(pT)]-cellulose chromatography. This RNA has been translated in both the wheat germ and reticulocyte lysate in vitro translation systems with greater than 50% of the synthesized protein being α-amylase. The mRNA for α-amylase has been further purified by dimethylsulfoxide-formamide-sucrose density gradient centrifugation and by gel electrophoresis. By these methods, its molecular weight has been determined to be 580,000.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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