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. 1980 May;65(5):1009–1013. doi: 10.1104/pp.65.5.1009

Cell Wall Metabolism in Ripening Fruit

I. CELL WALL CHANGES IN RIPENING `BARTLETT' PEARS 1

Ahmed Elrayah Ahmed 1,2, John M Labavitch 1
PMCID: PMC440466  PMID: 16661275

Abstract

`Bartlett' pear (Pyrus communis) fruits were picked at the mature, green stage and ripened at 20 C. Fruits at different stages of ripeness (based on flesh firmness) were homogenized, and the sugar and uronic acid contents of cell wall and soluble polysaccharides were determined. Substantial amounts of galacturonic acid and arabinose were lost from the wall fraction as the fruit ripened. Most of this cell wall material was recovered, in an 80% (volume/volume) ethanol-insoluble form, from the soluble fraction of tissue homogenates. Structural analysis of ethanol-precipitable material indicates that it is an acidic (pectic) polymer-bearing side groups containing variously-linked arabinosyl residues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
  2. DISCHE Z. Qualitative and quantitative colorimetric determination of heptoses. J Biol Chem. 1953 Oct;204(2):983–997. [PubMed] [Google Scholar]
  3. HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
  4. ISHERWOOD F. A., JERMYN M. A. Changes in the cell wall of the pear during ripening. Biochem J. 1956 Sep;64(1):123–132. doi: 10.1042/bj0640123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Maxie E. C., Eaks I. L., Sommer N. F., Rae H. L., El-Batal S. Effect of Gamma Radiation on Rate of Ethylene and Carbon Dioxide Evolution by Lemon Fruit. Plant Physiol. 1965 May;40(3):407–409. doi: 10.1104/pp.40.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sandford P. A., Conrad H. E. The structure of the Aerobacter aerogenes A3(S1) polysaccharide. I. A reexamination using improved procedures for methylation analysis. Biochemistry. 1966 May;5(5):1508–1517. doi: 10.1021/bi00869a009. [DOI] [PubMed] [Google Scholar]
  7. Talmadge K. W., Keegstra K., Bauer W. D., Albersheim P. The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides. Plant Physiol. 1973 Jan;51(1):158–173. doi: 10.1104/pp.51.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Updegraff D. M. Semimicro determination of cellulose in biological materials. Anal Biochem. 1969 Dec;32(3):420–424. doi: 10.1016/s0003-2697(69)80009-6. [DOI] [PubMed] [Google Scholar]
  9. Wallner S. J., Bloom H. L. Characteristics of tomato cell wall degradation in vitro: implications for the study of fruit-softening enzymes. Plant Physiol. 1977 Aug;60(2):207–210. doi: 10.1104/pp.60.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]

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