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. 1980 Jun;65(6):1053–1057. doi: 10.1104/pp.65.6.1053

Labeling and Isolation of Plasma Membranes from Corn Leaf Protoplasts 1

David S Perlin 1, Roger M Spanswick 1
PMCID: PMC440479  PMID: 16661329

Abstract

A plasma membrane-enriched fraction has been isolated from corn leaf mesophyll protoplasts and its identity confirmed with the aid of an external label, diazotized [125I]iodosulfanilic acid. Gentle cell disruption enabled internal organelles to be maintained intact and thus facilitated separation from the plasma membrane. The plasma membrane-enriched fraction was devoid of chloroplast or mitochondrial markers, whereas markers for the endoplasmic reticulum and golgi indicated minimal contamination. The highly enriched plasma membrane fraction contained a Mg2+-dependent, K+-stimulated ATPase with a pH optimum near neutrality. The position of the membranes on sucrose density gradients indicates that the plasma membranes have characteristics similar to other plasma membrane fractions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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