Figure 1.
Enhanced suppression of established Eμ-myc 4242 tumor growth in mice treated with a combination of α-GalCer-loaded tumor vaccine and anti-4–1BB antibody. (A) C57BL/6 wild-type (WT) mice were challenged with 1 × 105 GFP-expressing Eμ-myc 4242 tumor cells and then treated with single vaccination and/or anti-4–1BB mAb on the days indicated. (B) Mean ± SEM (n = 4 per group) Eμ-myc 4242 tumor burden in blood after designated treatments. (C) Overall survival of mice after receiving the indicated treatments. **P < 0.01; *P < 0.05, log-rank test. (D) Mice that had previously been treated with vaccine + anti-4–1BB mAb and showed tumor-free survival of at least 75 d were re-challenged with 1 × 105 Eμ-myc 4242 tumor cells and overall survival is shown compared to naive mice that received an equivalent number of tumor cells as a primary challenge (n = 6–8). **P < 0.01, log-rank test. Representative data from 3 independent experiments is shown for A–C. Pooled data from 2 independent experiments are shown for D.