Abstract
The relation between the composition of the phospholipid molecular species in a cell membrane and the velocity of protoplast fusion was studied using cells cultured at a low temperature (10 C). Cells cultured at a low temperature contained larger proportions of phospholipids of low phase transition point, the 1,2-dilinoleoyl-type, than those cultured at a normal temperature (25 C). When treated with polyethylene glycol 6000, protoplasts from cells cultured at 10 C fused and progressed to the fused sphere stage more rapidly than did those from cells cultured at 25 C.
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- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Carlson P. S., Smith H. H., Dearing R. D. Parasexual interspecific plant hybridization. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2292–2294. doi: 10.1073/pnas.69.8.2292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kagawa T., Lord J. M., Beevers H. The origin and turnover of organelle membranes in castor bean endosperm. Plant Physiol. 1973 Jan;51(1):61–65. doi: 10.1104/pp.51.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keller W. A., Melchers G. The effect of high pH and calcium on tobacco leaf protoplast fusion. Z Naturforsch C. 1973 Nov-Dec;28(11):737–741. doi: 10.1515/znc-1973-11-1215. [DOI] [PubMed] [Google Scholar]
- Kito M., Ishinaga M., Nishihara M., Kato M., Sawada S. Metabolism of the phosphatidylglycerol molecular species in Escherichia coli. Eur J Biochem. 1975 May;54(1):55–63. doi: 10.1111/j.1432-1033.1975.tb04113.x. [DOI] [PubMed] [Google Scholar]
- Morré D. J. In vivo incorporation of radioactive metabolites by Golgi apparatus and other cell fractions of onion stem. Plant Physiol. 1970 Jun;45(6):791–799. doi: 10.1104/pp.45.6.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishihara M., Kimura K., Izui K., Ishinaga M., Kato M. Phosphatidylethanolamine molecular species of fatty acid auxotroph of Escherichia coli grown with elaidate. Biochim Biophys Acta. 1975 Nov 21;409(2):212–217. [PubMed] [Google Scholar]