Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1980 Jun;65(6):1099–1102. doi: 10.1104/pp.65.6.1099

Protoplast Fusion

EFFECT OF LOW TEMPERATURE ON THE MEMBRANE FLUIDITY OF CULTURED CELLS

Yasuyuki Yamada 1, Yasuhiro Hara 1, Hiroaki Katagi 1, Mitsugi Senda 1
PMCID: PMC440489  PMID: 16661339

Abstract

The relation between the composition of the phospholipid molecular species in a cell membrane and the velocity of protoplast fusion was studied using cells cultured at a low temperature (10 C). Cells cultured at a low temperature contained larger proportions of phospholipids of low phase transition point, the 1,2-dilinoleoyl-type, than those cultured at a normal temperature (25 C). When treated with polyethylene glycol 6000, protoplasts from cells cultured at 10 C fused and progressed to the fused sphere stage more rapidly than did those from cells cultured at 25 C.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Carlson P. S., Smith H. H., Dearing R. D. Parasexual interspecific plant hybridization. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2292–2294. doi: 10.1073/pnas.69.8.2292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kagawa T., Lord J. M., Beevers H. The origin and turnover of organelle membranes in castor bean endosperm. Plant Physiol. 1973 Jan;51(1):61–65. doi: 10.1104/pp.51.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Keller W. A., Melchers G. The effect of high pH and calcium on tobacco leaf protoplast fusion. Z Naturforsch C. 1973 Nov-Dec;28(11):737–741. doi: 10.1515/znc-1973-11-1215. [DOI] [PubMed] [Google Scholar]
  5. Kito M., Ishinaga M., Nishihara M., Kato M., Sawada S. Metabolism of the phosphatidylglycerol molecular species in Escherichia coli. Eur J Biochem. 1975 May;54(1):55–63. doi: 10.1111/j.1432-1033.1975.tb04113.x. [DOI] [PubMed] [Google Scholar]
  6. Morré D. J. In vivo incorporation of radioactive metabolites by Golgi apparatus and other cell fractions of onion stem. Plant Physiol. 1970 Jun;45(6):791–799. doi: 10.1104/pp.45.6.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nishihara M., Kimura K., Izui K., Ishinaga M., Kato M. Phosphatidylethanolamine molecular species of fatty acid auxotroph of Escherichia coli grown with elaidate. Biochim Biophys Acta. 1975 Nov 21;409(2):212–217. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES