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. 1980 Jun;65(6):1224–1228. doi: 10.1104/pp.65.6.1224

Incorporation and Degradation of 14C and 3H-labeled Thymidine by Sugarcane Cells in Suspension Culture 1,2

Stanley M Lesley 1,2, Andrew Maretzki 1,2, Louis G Nickell 1,2,3
PMCID: PMC440515  PMID: 16661365

Abstract

Sugarcane cells growing in suspension culture degrade exogenous thymidine, releasing thymine. Thymine is not utilized for DNA synthesis. Thymine is rapidly catabolized to β-aminoisobutyric acid which is found within the cell. Thymidine in the medium is used for DNA synthesis. The label of [2-14C]thymidine is lost as 14CO2, but the label of [3H]methylthymidine is found in the cell as [3H]β-aminoisobutyric acid, some of which is used for the synthesis of other cell components. The degradation of thymidine can be partially inhibited by addition of certain substituted pyrimidines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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