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. 2015 Apr 14;145(1):150–160. doi: 10.1111/imm.12435

Figure 4.

Figure 4

Litomosoides sigmodontis infection increases suppressive CTLA-4+ CD4+ cells during Escherichia coli challenge. (a) Gating strategy to identify splenic CD4+ CD25+ FoxP3+ regulatory T cells and CD4+ CTLA-4+ T cells by flow cytometry. (b) Frequency and (c) total numbers of CD4+ Foxp3+ T cells and (d) frequency and (e) total numbers of CTLA-4+ CD4+ T cells. (f) Mean fluorescence intensity of CTLA-4 on CD4+ T cells. Spleen cells were analysed from L. sigmodontis-infected (L.s.) and uninfected animals before, 24 hr and 6 days post E. coli challenge (uninfected n = 6; L.s. n = 4; uninfected + E. coli n = 6; L.s. + E. coli n = 6 per point of time). One representative of two independent experiments is shown. Data are shown as mean ± SEM and were analysed for statistical significance by Mann–Whitney U-test. *P < 0·05; **P < 0·01.