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. 1980 Jul;66(1):93–96. doi: 10.1104/pp.66.1.93

Purification and Some Properties of Cell Wall-bound Invertases from Sugar Beet Seedlings and Aged Slices of Mature Roots

Hiroshi Masuda 1, Shiro Sugawara 1
PMCID: PMC440538  PMID: 16661402

Abstract

Cell wall-bound invertases (EC 3.2.1.26) from both sugar beet seedlings and aged slices of mature roots were purified to homogeneity separately with CM-cellulose chromatography and Bio-Gel P-150 gel filtrations. The enzymes behaved similarly throughout the purification procedures. The purified enzymes are identical as characterized by specific activity, gel electrophoretic mobility, Km for sucrose and raffinose (1.33 and 4.0 millimolar, respectively), mobility on Bio-Gel P-150 (molecular weight 28,000), optimum pH (4.6 to 5.0), optimum temperature, and dependence on NaCl concentration for insolubilization by DNA. The results suggest that the enzymes may be encoded for by the same structural gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EDELMAN J., HALL M. A. ENZYME FORMATION IN HIGHER-PLANT TISSUES. DEVELOPMENT OF INVERTASE AND ASCORBATE-OXIDASE ACTIVITIES IN MATURE STORAGE TISSUE OF HELIANTHUS TUBEROSUS L. Biochem J. 1965 May;95:403–410. doi: 10.1042/bj0950403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Giaquinta R. T. Sucrose translocation and storage in the sugar beet. Plant Physiol. 1979 May;63(5):828–832. doi: 10.1104/pp.63.5.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hatch M. D., Glasziou K. T. Sugar Accumulation Cycle in Sugar Cane. II. Relationship of Invertase Activity to Sugar Content & Growth Rate in Storage Tissue of Plants Grown in Controlled Environments. Plant Physiol. 1963 May;38(3):344–348. doi: 10.1104/pp.38.3.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Matsushita K., Uritani I. Change in invertase activity of sweet potato in response to wounding and purification and properties of its invertases. Plant Physiol. 1974 Jul;54(1):60–66. doi: 10.1104/pp.54.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
  7. SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]

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