Abstract
15NH4+ and [15N](amide)-glutamine externally supplied to detached nodules from soybean plants (cv. Tamanishiki) were incorporated within nodule tissues by vacuum infiltration and metabolized to various nitrogen compounds during 60 minutes of incubation time. In the case of 15NH4+ - feeding, the 15N abundance ratio was highest in the amide nitrogen of glutamine, followed by glutamate and the amide nitrogen of asparagine. In 15N content (micrograms excess 15N), the amide nitrogen of asparagine was most highly enriched after 60 minutes. 15NH4+ was also appreciably assimilated into alanine.
When [15N](amide)-glutamine was fed to detached nodules, 15N was almost entirely in the amide nitrogen of asparagine. The result indicates the presence of active glutamine-linked asparagine synthesizing systems in soybean root nodules.
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- Fujihara S., Yamaguchi M. Effects of Allopurinol [4-Hydroxypyrazolo(3,4-d)Pyrimidine] on the Metabolism of Allantoin in Soybean Plants. Plant Physiol. 1978 Jul;62(1):134–138. doi: 10.1104/pp.62.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Streeter J. G. Allantoin and Allantoic Acid in Tissues and Stem Exudate from Field-grown Soybean Plants. Plant Physiol. 1979 Mar;63(3):478–480. doi: 10.1104/pp.63.3.478. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Streeter J. G. Asparaginase and asparagine transaminase in soybean leaves and root nodules. Plant Physiol. 1977 Aug;60(2):235–239. doi: 10.1104/pp.60.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Streeter J. G. In vivo and in vitro studies on asparagine biosynthesis in soybean seedlings. Arch Biochem Biophys. 1973 Aug;157(2):613–624. doi: 10.1016/0003-9861(73)90681-4. [DOI] [PubMed] [Google Scholar]
- Stulen I., Oaks A. Asparagine synthetase in corn roots. Plant Physiol. 1977 Nov;60(5):680–683. doi: 10.1104/pp.60.5.680. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wong P. P., Evans H. J. Poly-beta-hydroxybutyrate Utilization by Soybean (Glycine max Merr.) Nodules and Assessment of Its Role in Maintenance of Nitrogenase Activity. Plant Physiol. 1971 Jun;47(6):750–755. doi: 10.1104/pp.47.6.750. [DOI] [PMC free article] [PubMed] [Google Scholar]