Figure 1.
(A) Schematic showing the seeding of hiPSC-ECs (7 × 104 cells/cm2) onto fibronectin-coated Biopore membranes (2.5 cm × 2.8 cm) and placement into cylindrical vessel chamber, indicated by the arrow (B) Schematic showing cross-section of the vessel chamber (OD: 8.66 mm) and assembled bioreactor with vessel chamber inside, connected to flow pump and medium flowing through tubing in direction of red arrows. (C) Physical model of vessel chamber with arrow pointing to placement in assembled glass bioreactor.