Abstract
Potato tuber mitochondria oxidizing malate respond to NAD+ addition with increased oxidation rates, whereas mung bean hypocotyl mitochondria do not. This is traced to a low endogenous content of NAD+ in potato mitochondria, which prove to take up added NAD+. This mechanism concentrates NAD+ in the matrix space. Analyses for oxaloacetate and pyruvate (with pyruvate dehydrogenase blocked) are consistent with regulation of malate oxidation by the internal NAD+/NADH ratio.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abou-Khalil S., Hanson J. B. Energy-linked Adenosine Diphosphate Accumulation by Corn Mitochondria: I. General Characteristics and Effect of Inhibitors. Plant Physiol. 1979 Aug;64(2):276–280. doi: 10.1104/pp.64.2.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Abou-Khalil S., Hanson J. B. Net adenosine diphosphate accumulation in mitochondria. Arch Biochem Biophys. 1977 Oct;183(2):581–587. doi: 10.1016/0003-9861(77)90393-9. [DOI] [PubMed] [Google Scholar]
- Bowman E. J., Ikuma H. Regulation of Malate Oxidation in Isolated Mung Bean Mitochondria: II. Role of Adenylates. Plant Physiol. 1976 Sep;58(3):438–446. doi: 10.1104/pp.58.3.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brunton C. J., Palmer J. M. Pathways for the oxidation of malate and reduced pyridine nucleotide by wheat mitochondria. Eur J Biochem. 1973 Nov 1;39(1):283–291. doi: 10.1111/j.1432-1033.1973.tb03125.x. [DOI] [PubMed] [Google Scholar]
- Chance B., Ernster L., Garland P. B., Lee C. P., Light P. A., Ohnishi T., Ragan C. I., Wong D. Flavoproteins of the mitochondrial respiratory chain. Proc Natl Acad Sci U S A. 1967 May;57(5):1498–1505. doi: 10.1073/pnas.57.5.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coleman J. O., Palmer J. M. The oxidation of malate by isolated plant mitochondria. Eur J Biochem. 1972 Apr 24;26(4):499–509. doi: 10.1111/j.1432-1033.1972.tb01792.x. [DOI] [PubMed] [Google Scholar]
- Day D. A., Wiskich J. T. The Effect of Exogenous Nicotinamide Adenine Dinucleotide on the Oxidation of Nicotinamide Adenine Dinucleotide-linked Substrates by Isolated Plant Mitochondria. Plant Physiol. 1974 Sep;54(3):360–363. doi: 10.1104/pp.54.3.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Day D. A., Wiskich J. T. The oxidation of malate and exogenous reduced nicotinamide adenine dinucleotide by isolated plant mitochondria. Plant Physiol. 1974 Jan;53(1):104–109. doi: 10.1104/pp.53.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Douce R., Christensen E. L., Bonner W. D., Jr Preparation of intaintact plant mitochondria. Biochim Biophys Acta. 1972 Aug 17;275(2):148–160. doi: 10.1016/0005-2728(72)90035-7. [DOI] [PubMed] [Google Scholar]
- Douce R., Mannella C. A., Bonner W. D., Jr The external NADH dehydrogenases of intact plant mitochondria. Biochim Biophys Acta. 1973 Jan 18;292(1):105–116. doi: 10.1016/0005-2728(73)90255-7. [DOI] [PubMed] [Google Scholar]
- LaNoue K. F., Bryla J., Williamson J. R. Feedback interactions in the control of citric acid cycle activity in rat heart mitochondria. J Biol Chem. 1972 Feb 10;247(3):667–679. [PubMed] [Google Scholar]
- Macrae A. R., Moorhouse R. The oxidation of malate by mitochondria isolated from cauliflower buds. Eur J Biochem. 1970 Sep;16(1):96–102. doi: 10.1111/j.1432-1033.1970.tb01058.x. [DOI] [PubMed] [Google Scholar]
- Neuburger M., Douce R. Effect of bicarbonate and oxaloacetate on malate oxidation by spinach leaf mitochondria. Biochim Biophys Acta. 1980 Feb 8;589(2):176–189. doi: 10.1016/0005-2728(80)90036-5. [DOI] [PubMed] [Google Scholar]
- Pfaff E., Heldt H. W., Klingenberg M. Adenine nucleotide translocation of mitochondria. Kinetics of the adenine nucleotide exchange. Eur J Biochem. 1969 Oct;10(3):484–493. doi: 10.1111/j.1432-1033.1969.tb00715.x. [DOI] [PubMed] [Google Scholar]
- Wedding R. T., Black M. K., Pap D. Malate Dehydrogenase and NAD Malic Enzyme in the Oxidation of Malate by Sweet Potato Mitochondria. Plant Physiol. 1976 Dec;58(6):740–743. doi: 10.1104/pp.58.6.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wiskich J. T., Bonner W. D. Preparation and Properties of Sweet Potato Mitochondria. Plant Physiol. 1963 Sep;38(5):594–604. doi: 10.1104/pp.38.5.594. [DOI] [PMC free article] [PubMed] [Google Scholar]