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. 1980 Sep;66(3):494–499. doi: 10.1104/pp.66.3.494

X-ray Analysis Studies of Elements Stored in Protein Body Globoid Crystals of Triticum Grains 1

John N A Lott 1, Ernest Spitzer 1
PMCID: PMC440660  PMID: 16661462

Abstract

Energy-dispersive x-ray analysis was used to investigate the elemental storage within protein bodies, specifically the globoid crystals, in grains of wheat. Areas of the grain investigated included various parts of the embryo, the aleurone layer plus starchy endosperm near the embryo and the aleurone layer plus starchy endosperm farthest from the embryo. Variations did occur grain-to-grain, cell-to-cell and, in certain regions, intracellularly. No protein bodies with electron-dense globoid crystals were found in the starchy endosperm. Generally globoid crystals contained P, K, and Mg in all areas investigated. Globoid crystals from the aleurone layer farthest from the embryo on occasion contained Ca, whereas aleurone globoid crystals near the embryo sometimes contained Fe. In most of the embryo regions examined, a few globoid crystals contained Ca along with P, K, and Mg. No specific pattern to the Ca distribution could be found. Welldefined elemental distribution occurred with Mn. Manganese was found only in globoid crystals located in the base and midregions of the stele in the radicle. Thus, in wheat there is some specific distribution of minerals dependent upon cell type and/or position in the grain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ergle D. R., Guinn G. Phosphorus Compounds of Cotton Embryos and Their Changes during Germination. Plant Physiol. 1959 Jul;34(4):476–481. doi: 10.1104/pp.34.4.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Lott J. N., Greenwood J. S., Vollmer C. M. Energy-dispersive x-Ray Analysis of Phosphorus, Potassium, Magnesium, and Calcium in Globoid Crystals in Protein Bodies from Different Regions of Cucurbita maxima Embryos. Plant Physiol. 1978 Jun;61(6):984–988. doi: 10.1104/pp.61.6.984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lott J. N. Protein Body Composition in Cuburbita maxima Cotyledons as Determined by Energy Dispersive X-Ray Analysis. Plant Physiol. 1975 May;55(5):913–916. doi: 10.1104/pp.55.5.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lott J. N., Spitzer E., Vollmer C. M. Calcium distribution in globoid crystals of cucurbita cotyledon protein bodies. Plant Physiol. 1979 May;63(5):847–851. doi: 10.1104/pp.63.5.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lott J. N., Vollmer C. M. Composition of globoid crystals from embryo protein bodies in five species of cucurbita. Plant Physiol. 1979 Feb;63(2):307–311. doi: 10.1104/pp.63.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lui N. S., Altschul A. M. Isolation of globoids from cottonseed aleurone grain. Arch Biochem Biophys. 1967 Sep;121(3):678–684. doi: 10.1016/0003-9861(67)90053-7. [DOI] [PubMed] [Google Scholar]
  7. Nahapetian A., Bassiri A. Variations in concentrations and interrelationships of phytate, phosphorus, magnesium, calcium, zinc, and iron in wheat varieties during two years. J Agric Food Chem. 1976 Sep-Oct;24(5):947–950. doi: 10.1021/jf60207a029. [DOI] [PubMed] [Google Scholar]
  8. Swift J. G., Buttrose M. S. Freeze-etch studies of protein bodies in wheat scutellum. J Ultrastruct Res. 1972 Aug;40(3):378–390. doi: 10.1016/s0022-5320(72)90108-6. [DOI] [PubMed] [Google Scholar]
  9. Tanaka K., Yoshida T., Asada K., Kasai Z. Subcellular particles isolated from aleurone layer of rice seeds. Arch Biochem Biophys. 1973 Mar;155(1):136–143. doi: 10.1016/s0003-9861(73)80016-5. [DOI] [PubMed] [Google Scholar]

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