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. 1980 Sep;66(3):500–504. doi: 10.1104/pp.66.3.500

Phytochrome Modification and Light-enhanced, In Vivo-induced Phytochrome Pelletability 1,2

Maury L Boeshore 1, Lee H Pratt 1
PMCID: PMC440661  PMID: 16661463

Abstract

Phytochrome that was induced by red irradiation in vivo to pellet with subcellular material and that was released from the pellet by removal of divalent cations exhibited altered characteristics. Compared to phytochrome extracted in a soluble red-absorbing form from etiolated tissue, pelleted and released phytochrome, which was also assayed in the red-absorbing form even though pelleted in the far-red-absorbing form, showed 50% greater micro complement fixation activity, eluted closer to the void volume of a Sephadex G-200 column, and electrophoresed more slowly on sodium dodecyl sulfate-polyacrylamide gels. Data presented here document that phytochrome pelleted in the far-red-absorbing form differs from soluble phytochrome extracted from nonirradiated tissue. These data, however, do not permit the conclusion that there is a causal relationship between pelletability and phytochrome modification.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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